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BODIPY-Based Fluorometric Sensor for the Simultaneous Determination of Cys, Hcy, and GSH in Human Serum
Jia, Ming-Yan1,3; Niu, Li-Ya2; Zhang, Yu1; Yang, Qing-Zheng2; Tung, Chen-Ho2; Guan, Ya-Feng1; Feng, Liang1
关键词Bodipy Cysteine Homocysteine Glutathione Serum
刊名ACS APPLIED MATERIALS & INTERFACES
2015-03-18
DOI10.1021/acsami.5b00122
7期:10页:5907-5914
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Technology
类目[WOS]Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
研究领域[WOS]Science & Technology - Other Topics ; Materials Science
关键词[WOS]RATIOMETRIC FLUORESCENT SENSOR ; DIFFERENT EMISSION CHANNELS ; HIGHLY SELECTIVE DETECTION ; LIVING CELLS ; GLUTATHIONE-REDUCTASE ; TOTAL HOMOCYSTEINE ; TOTAL CYSTEINE ; PROBE ; PLASMA ; THIOLS
英文摘要Cysteine (Cys), homocysteine (Hey), and glutathione (GSH) are interconbected and play essential roles for regulating the redox balance of biological processes. However, finding a simple and effective method for the simultaneous determination for these three biothiols in biological systems is always a challenge. In this work, we report a method for the simultaneous quantitative determination of three biothiols in a mixture using a monochlorinated boron dipyrromethene (BODIPY)-based fluorometric sensor. At a specified period of time, after reacting with excess sensor, Hcy and GSH form predominantly sulfur-substituted BODIPY, while Cys generates sulfur-amino-diBODIPY due to a fast substitution-rearrangement substitution reaction. A significant difference in polarities of these respective major products simplifies their separation by TLC, thus leading to the simultaneous determination of Cys, Hey, and GSH readily. The sensor was successfully applied for the simultaneous quantitative detection of three biothiols in human serum, and the results were in good agreement with those obtained via high performance liquid chromatography (HPLC).
语种英语
WOS记录号WOS:000351420300034
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/146089
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Key Lab Photochem Convers & Optoelect Mat, Tech Inst Phys & Chem, Beijing 100191, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Jia, Ming-Yan,Niu, Li-Ya,Zhang, Yu,et al. BODIPY-Based Fluorometric Sensor for the Simultaneous Determination of Cys, Hcy, and GSH in Human Serum[J]. ACS APPLIED MATERIALS & INTERFACES,2015,7(10):5907-5914.
APA Jia, Ming-Yan.,Niu, Li-Ya.,Zhang, Yu.,Yang, Qing-Zheng.,Tung, Chen-Ho.,...&Feng, Liang.(2015).BODIPY-Based Fluorometric Sensor for the Simultaneous Determination of Cys, Hcy, and GSH in Human Serum.ACS APPLIED MATERIALS & INTERFACES,7(10),5907-5914.
MLA Jia, Ming-Yan,et al."BODIPY-Based Fluorometric Sensor for the Simultaneous Determination of Cys, Hcy, and GSH in Human Serum".ACS APPLIED MATERIALS & INTERFACES 7.10(2015):5907-5914.
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