DICP OpenIR
BODIPY-Based Fluorometric Sensor for the Simultaneous Determination of Cys, Hcy, and GSH in Human Serum
Jia, Ming-Yan1,3; Niu, Li-Ya2; Zhang, Yu1; Yang, Qing-Zheng2; Tung, Chen-Ho2; Guan, Ya-Feng1; Feng, Liang1
KeywordBodipy Cysteine Homocysteine Glutathione Serum
Source PublicationACS APPLIED MATERIALS & INTERFACES
2015-03-18
DOI10.1021/acsami.5b00122
Volume7Issue:10Pages:5907-5914
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
WOS SubjectNanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS Research AreaScience & Technology - Other Topics ; Materials Science
WOS KeywordRATIOMETRIC FLUORESCENT SENSOR ; DIFFERENT EMISSION CHANNELS ; HIGHLY SELECTIVE DETECTION ; LIVING CELLS ; GLUTATHIONE-REDUCTASE ; TOTAL HOMOCYSTEINE ; TOTAL CYSTEINE ; PROBE ; PLASMA ; THIOLS
AbstractCysteine (Cys), homocysteine (Hey), and glutathione (GSH) are interconbected and play essential roles for regulating the redox balance of biological processes. However, finding a simple and effective method for the simultaneous determination for these three biothiols in biological systems is always a challenge. In this work, we report a method for the simultaneous quantitative determination of three biothiols in a mixture using a monochlorinated boron dipyrromethene (BODIPY)-based fluorometric sensor. At a specified period of time, after reacting with excess sensor, Hcy and GSH form predominantly sulfur-substituted BODIPY, while Cys generates sulfur-amino-diBODIPY due to a fast substitution-rearrangement substitution reaction. A significant difference in polarities of these respective major products simplifies their separation by TLC, thus leading to the simultaneous determination of Cys, Hey, and GSH readily. The sensor was successfully applied for the simultaneous quantitative detection of three biothiols in human serum, and the results were in good agreement with those obtained via high performance liquid chromatography (HPLC).
Language英语
WOS IDWOS:000351420300034
Citation statistics
Cited Times:84[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/146089
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Key Lab Photochem Convers & Optoelect Mat, Tech Inst Phys & Chem, Beijing 100191, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Jia, Ming-Yan,Niu, Li-Ya,Zhang, Yu,et al. BODIPY-Based Fluorometric Sensor for the Simultaneous Determination of Cys, Hcy, and GSH in Human Serum[J]. ACS APPLIED MATERIALS & INTERFACES,2015,7(10):5907-5914.
APA Jia, Ming-Yan.,Niu, Li-Ya.,Zhang, Yu.,Yang, Qing-Zheng.,Tung, Chen-Ho.,...&Feng, Liang.(2015).BODIPY-Based Fluorometric Sensor for the Simultaneous Determination of Cys, Hcy, and GSH in Human Serum.ACS APPLIED MATERIALS & INTERFACES,7(10),5907-5914.
MLA Jia, Ming-Yan,et al."BODIPY-Based Fluorometric Sensor for the Simultaneous Determination of Cys, Hcy, and GSH in Human Serum".ACS APPLIED MATERIALS & INTERFACES 7.10(2015):5907-5914.
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