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题名: BODIPY-Based Fluorometric Sensor for the Simultaneous Determination of Cys, Hcy, and GSH in Human Serum
作者: Jia, Ming-Yan1, 3;  Niu, Li-Ya2;  Zhang, Yu1;  Yang, Qing-Zheng2;  Tung, Chen-Ho2;  Guan, Ya-Feng1;  Feng, Liang1
关键词: BODIPY ;  cysteine ;  homocysteine ;  glutathione ;  serum
刊名: ACS APPLIED MATERIALS & INTERFACES
发表日期: 2015-03-18
DOI: 10.1021/acsami.5b00122
卷: 7, 期:10, 页:5907-5914
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Nanoscience & Nanotechnology ;  Materials Science, Multidisciplinary
研究领域[WOS]: Science & Technology - Other Topics ;  Materials Science
英文摘要: Cysteine (Cys), homocysteine (Hey), and glutathione (GSH) are interconbected and play essential roles for regulating the redox balance of biological processes. However, finding a simple and effective method for the simultaneous determination for these three biothiols in biological systems is always a challenge. In this work, we report a method for the simultaneous quantitative determination of three biothiols in a mixture using a monochlorinated boron dipyrromethene (BODIPY)-based fluorometric sensor. At a specified period of time, after reacting with excess sensor, Hcy and GSH form predominantly sulfur-substituted BODIPY, while Cys generates sulfur-amino-diBODIPY due to a fast substitution-rearrangement substitution reaction. A significant difference in polarities of these respective major products simplifies their separation by TLC, thus leading to the simultaneous determination of Cys, Hey, and GSH readily. The sensor was successfully applied for the simultaneous quantitative detection of three biothiols in human serum, and the results were in good agreement with those obtained via high performance liquid chromatography (HPLC).
关键词[WOS]: RATIOMETRIC FLUORESCENT SENSOR ;  DIFFERENT EMISSION CHANNELS ;  HIGHLY SELECTIVE DETECTION ;  LIVING CELLS ;  GLUTATHIONE-REDUCTASE ;  TOTAL HOMOCYSTEINE ;  TOTAL CYSTEINE ;  PROBE ;  PLASMA ;  THIOLS
语种: 英语
WOS记录号: WOS:000351420300034
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/146089
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Key Lab Photochem Convers & Optoelect Mat, Tech Inst Phys & Chem, Beijing 100191, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Jia, Ming-Yan,Niu, Li-Ya,Zhang, Yu,et al. BODIPY-Based Fluorometric Sensor for the Simultaneous Determination of Cys, Hcy, and GSH in Human Serum[J]. ACS APPLIED MATERIALS & INTERFACES,2015,7(10):5907-5914.
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