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Enhancement of L-2-haloacid dehalogenase expression in Pseudomonas stutzeri DEH138 based on the different substrate specificity between dehalogenase-producing bacteria and their dehalogenases
Wang, Yayue1,2; Xin, Yanjuan1; Cao, Xupeng1; Xue, Song1
关键词Pseudomonas Stutzeri Inducers 2-haloacid Dehalogenase Production
刊名WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
2015-04-01
DOI10.1007/s11274-015-1817-2
31期:4页:669-673
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]SPONGE HYMENIACIDON-PERLEVIS ; 2-HALOACID DEHALOGENASE ; 1,2-DICHLOROETHANE ; PURIFICATION ; DEGRADATION ; ACIDS
英文摘要There was no direct correlation in substrate specificity between the metabolism of Pseudomonas stutzeri DEH138 and its corresponding dehalogenase. Dehalogenase substrates that could be dehalogenated might not be degraded by DEH138 or vice versa. Basing on this, different approaches to enhance L-2-haloacid dehalogenase (L-DEX) production in DEH138 via the combination of non-halogenated compounds with different inducers were applied. The optimum approach to obtain more L-DEX from DEH138 was the combination of DL-lactate and DL-2-chlorobutyrate, with 5.7-fold greater production and 11.7-fold greater productivity of the enzyme after optimization.
语种英语
WOS记录号WOS:000351294200012
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/146099
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioengn Grp, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Wang, Yayue,Xin, Yanjuan,Cao, Xupeng,et al. Enhancement of L-2-haloacid dehalogenase expression in Pseudomonas stutzeri DEH138 based on the different substrate specificity between dehalogenase-producing bacteria and their dehalogenases[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2015,31(4):669-673.
APA Wang, Yayue,Xin, Yanjuan,Cao, Xupeng,&Xue, Song.(2015).Enhancement of L-2-haloacid dehalogenase expression in Pseudomonas stutzeri DEH138 based on the different substrate specificity between dehalogenase-producing bacteria and their dehalogenases.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,31(4),669-673.
MLA Wang, Yayue,et al."Enhancement of L-2-haloacid dehalogenase expression in Pseudomonas stutzeri DEH138 based on the different substrate specificity between dehalogenase-producing bacteria and their dehalogenases".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 31.4(2015):669-673.
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