中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Enhancement of L-2-haloacid dehalogenase expression in Pseudomonas stutzeri DEH138 based on the different substrate specificity between dehalogenase-producing bacteria and their dehalogenases
作者: Wang, Yayue1, 2;  Xin, Yanjuan1;  Cao, Xupeng1;  Xue, Song1
关键词: Pseudomonas stutzeri ;  Inducers ;  2-Haloacid dehalogenase ;  Production
刊名: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
发表日期: 2015-04-01
DOI: 10.1007/s11274-015-1817-2
卷: 31, 期:4, 页:669-673
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: There was no direct correlation in substrate specificity between the metabolism of Pseudomonas stutzeri DEH138 and its corresponding dehalogenase. Dehalogenase substrates that could be dehalogenated might not be degraded by DEH138 or vice versa. Basing on this, different approaches to enhance L-2-haloacid dehalogenase (L-DEX) production in DEH138 via the combination of non-halogenated compounds with different inducers were applied. The optimum approach to obtain more L-DEX from DEH138 was the combination of DL-lactate and DL-2-chlorobutyrate, with 5.7-fold greater production and 11.7-fold greater productivity of the enzyme after optimization.
关键词[WOS]: SPONGE HYMENIACIDON-PERLEVIS ;  2-HALOACID DEHALOGENASE ;  1,2-DICHLOROETHANE ;  PURIFICATION ;  DEGRADATION ;  ACIDS
语种: 英语
WOS记录号: WOS:000351294200012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/146099
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioengn Grp, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Wang, Yayue,Xin, Yanjuan,Cao, Xupeng,et al. Enhancement of L-2-haloacid dehalogenase expression in Pseudomonas stutzeri DEH138 based on the different substrate specificity between dehalogenase-producing bacteria and their dehalogenases[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2015,31(4):669-673.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Wang, Yayue]'s Articles
 [Xin, Yanjuan]'s Articles
 [Cao, Xupeng]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Wang, Yayue]‘s Articles
 [Xin, Yanjuan]‘s Articles
 [Cao, Xupeng]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace