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题名: Enhanced Multi-phosphopeptide Enrichment and Nano LC-ESI-qTOE-MS Detection Strategy Using Click OEG-CD Matrix
作者: Zhao Yanyan1;  Wang Liheng2;  Guo Zhimou3;  Chi Xiaofei2;  Ma Xiaochi1;  Qi Yan1;  Fang Shinong1;  Li Xiuling3;  Liang Xinmiao3
关键词: Click OEG-CD[olio(ethylene glycol)(OEG) linked beta-cyclodextrin(CD)] matrix ;  Multi-phosphopeptide enrichment ;  MS detection
刊名: CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
发表日期: 2015-02-01
DOI: 10.1007/s40242-014-4179-7
卷: 31, 期:1, 页:44-52
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: Detection and determination of multi-phosphopeptides from protein digestion products are difficult due to the low abundance and ion-suppression effect arised from the existence of their high-abundance counterparts. Click OEG-CD[olio(ethylene glycol)(OEG) linked,beta-cyclodextrin(CD)] matrix has shown excellent performance in phosphopeptide enrichment. However, few multi-phosphopeptides were detected previously. In this investigation, an improved method aiming at enhancing the enrichment selectivity and mass spectrometry(MS) detection of multi-phosphopeptide was developed via optimizing the sample loading amount on per mg of matrix. Mass spectra were obtained on a Nano liquid chromatography-electrospray ionization-quadrupole time of flight-mass spectrometry (LC-ESI-qTOE-MS). The enrichment selectivity of double-phosphopeptide could be enhanced with the increase of loading amount on per mg of matrix when taking the mixture of mono-, double-, and non-phosphopeptides as probe. Furthermore, the multi-phosphopeptide enrichment selectivity was enhanced under the condition of optimized loading amount when taking the product of typtic digestion a-casein as test sample. When the loading amount was 573 pmol/mg matrix, up to 20 a-casein phosphopeptide signals(including 15 multi-phosphopeptides) were detected. The result is much better than that of our previous report. The reduction of ion-suppression effect arised from the existence of high-abundance non- or mono-phosphopeptides and the stronger interactions between multi-phosphopepides and the matrix were contributed to the result. The study could be helpful to the better utilization of Click OEG,CD matrix in the enrichment of multi-phosphopeptide from complex biosamples in the subsequent investigation.
关键词[WOS]: PHOSPHOPROTEOME ANALYSIS ;  PHOSPHORYLATION ANALYSIS ;  PROTEIN-PHOSPHORYLATION ;  SELECTIVE ENRICHMENT ;  CHROMATOGRAPHY ;  PEPTIDES
语种: 英语
WOS记录号: WOS:000351479100009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/146104
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Med Univ, Coll Pharm, Dalian 116044, Peoples R China
2.Second Hosp Dalian City, Dalian 116011, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Zhao Yanyan,Wang Liheng,Guo Zhimou,et al. Enhanced Multi-phosphopeptide Enrichment and Nano LC-ESI-qTOE-MS Detection Strategy Using Click OEG-CD Matrix[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2015,31(1):44-52.
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