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Enhanced Multi-phosphopeptide Enrichment and Nano LC-ESI-qTOE-MS Detection Strategy Using Click OEG-CD Matrix
Zhao Yanyan1; Wang Liheng2; Guo Zhimou3; Chi Xiaofei2; Ma Xiaochi1; Qi Yan1; Fang Shinong1; Li Xiuling3; Liang Xinmiao3
KeywordClick Oeg-Cd[Olio(Ethylene Glycol)(Oeg) Linked Beta-cyclodextrin(Cd)] Matrix Multi-phosphopeptide Enrichment Ms Detection
Source PublicationCHEMICAL RESEARCH IN CHINESE UNIVERSITIES
2015-02-01
DOI10.1007/s40242-014-4179-7
Volume31Issue:1Pages:44-52
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordPHOSPHOPROTEOME ANALYSIS ; PHOSPHORYLATION ANALYSIS ; PROTEIN-PHOSPHORYLATION ; SELECTIVE ENRICHMENT ; CHROMATOGRAPHY ; PEPTIDES
AbstractDetection and determination of multi-phosphopeptides from protein digestion products are difficult due to the low abundance and ion-suppression effect arised from the existence of their high-abundance counterparts. Click OEG-CD[olio(ethylene glycol)(OEG) linked,beta-cyclodextrin(CD)] matrix has shown excellent performance in phosphopeptide enrichment. However, few multi-phosphopeptides were detected previously. In this investigation, an improved method aiming at enhancing the enrichment selectivity and mass spectrometry(MS) detection of multi-phosphopeptide was developed via optimizing the sample loading amount on per mg of matrix. Mass spectra were obtained on a Nano liquid chromatography-electrospray ionization-quadrupole time of flight-mass spectrometry (LC-ESI-qTOE-MS). The enrichment selectivity of double-phosphopeptide could be enhanced with the increase of loading amount on per mg of matrix when taking the mixture of mono-, double-, and non-phosphopeptides as probe. Furthermore, the multi-phosphopeptide enrichment selectivity was enhanced under the condition of optimized loading amount when taking the product of typtic digestion a-casein as test sample. When the loading amount was 573 pmol/mg matrix, up to 20 a-casein phosphopeptide signals(including 15 multi-phosphopeptides) were detected. The result is much better than that of our previous report. The reduction of ion-suppression effect arised from the existence of high-abundance non- or mono-phosphopeptides and the stronger interactions between multi-phosphopepides and the matrix were contributed to the result. The study could be helpful to the better utilization of Click OEG,CD matrix in the enrichment of multi-phosphopeptide from complex biosamples in the subsequent investigation.
Language英语
WOS IDWOS:000351479100009
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/146104
Collection中国科学院大连化学物理研究所
Affiliation1.Dalian Med Univ, Coll Pharm, Dalian 116044, Peoples R China
2.Second Hosp Dalian City, Dalian 116011, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Zhao Yanyan,Wang Liheng,Guo Zhimou,et al. Enhanced Multi-phosphopeptide Enrichment and Nano LC-ESI-qTOE-MS Detection Strategy Using Click OEG-CD Matrix[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2015,31(1):44-52.
APA Zhao Yanyan.,Wang Liheng.,Guo Zhimou.,Chi Xiaofei.,Ma Xiaochi.,...&Liang Xinmiao.(2015).Enhanced Multi-phosphopeptide Enrichment and Nano LC-ESI-qTOE-MS Detection Strategy Using Click OEG-CD Matrix.CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,31(1),44-52.
MLA Zhao Yanyan,et al."Enhanced Multi-phosphopeptide Enrichment and Nano LC-ESI-qTOE-MS Detection Strategy Using Click OEG-CD Matrix".CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 31.1(2015):44-52.
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