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题名: Hypoxia combined with spheroid culture improves cartilage specific function in chondrocytes
作者: Shi, Yang1;  Ma, Jingyun1;  Zhang, Xu1;  Li, Hongjing2;  Jiang, Lei1;  Qin, Jianhua1
刊名: INTEGRATIVE BIOLOGY
发表日期: 2015
DOI: 10.1039/c4ib00273c
卷: 7, 期:3, 页:289-297
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
英文摘要: Controlling the chondrocyte phenotype and function in a physiologically relevant microenvironment remains a major challenge for cartilage repair in tissue engineering applications. This work presents a straightforward strategy to create a high throughput concave microwell array used for generating multicellular spheroids of chondrocytes and facilitating the maintenance of the articular chondrocyte phenotype and function by combining 3D spheroid culture with hypoxia. The polydimethylsiloxane (PDMS) concave microwells were simply produced from a concave SU-8 template fabricated using a soft-lithography approach and easily adopted for size-controlled spheroid culture. 3D spheroid culture was observed to facilitate the cartilage-specific phenotype and function maintenance as compared to 2D monolayer culture. Combining hypoxia with spheroid culture markedly increased the expressions of cartilage-specific collagen II and aggrecan at protein and mRNA levels. The hypoxia-inducible factor (HIF) signaling pathway was found to get involved in phenotype maintenance, metabolism and differentiation of chondrocytes by regulating HIF-1 alpha and HIF-2 alpha, respectively. The established approach provides a useful platform for a wide range of applications in the field of cartilage biology, stem cell research and high throughput 3D drug testing in cancer.
关键词[WOS]: ARTICULAR CHONDROCYTES ;  PHENOTYPIC STABILITY ;  MICROWELL ARRAYS ;  IN-VITRO ;  REDIFFERENTIATION ;  MATRIX ;  FABRICATION ;  ALGINATE ;  MODELS ;  3D
语种: 英语
WOS记录号: WOS:000351136500002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/146106
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 1, Dept Orthoped, Dalian 116023, Peoples R China

Recommended Citation:
Shi, Yang,Ma, Jingyun,Zhang, Xu,et al. Hypoxia combined with spheroid culture improves cartilage specific function in chondrocytes[J]. INTEGRATIVE BIOLOGY,2015,7(3):289-297.
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