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题名: Preparation and chromatographic evaluation of a newly designed steviol glycoside modified-silica stationary phase in hydrophilic interaction liquid chromatography and reversed phase liquid chromatography
作者: Liang, Tu1;  Fu, Qing1;  Shen, Aijin2;  Wang, Hui1;  Jin, Yu1;  Xin, Huaxia1;  Ke, Yanxiong1;  Guo, Zhimou2;  Liang, Xinmiao1, 2
关键词: Rebaudioside A ;  Stationary phase ;  Hydrophilic interaction liquid chromatography (HILIC) ;  Mixed-mode ;  Thiol-ene click chemistry
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2015-04-03
DOI: 10.1016/j.chroma.2015.02.019
卷: 1388, 页:110-118
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A diterpene glycoside compound, rebaudioside A (commonly abbreviated as RA), was immobilized onto porous silica surface through "thiol-ene" click chemistry strategy. The successful immobilization of the RA on the silica support was confirmed by FT-IR and elemental analysis. Chromatographic characteristics of the new stationary phase, named Click TE-RA, were evaluated by a set of diverse analytes such as carbohydrates, nucleosides, and organic acids in hydrophilic interaction liquid chromatography (HILIC) mode. The effects of water content, buffer pH and concentration were investigated and a typical HILIC retention feature of Click TE-RA was observed at high organic modifier content. The Click TE-RA stationary phase was further studied by a series of glycoside compounds. Tunable retention mechanisms from hydrophilic to hydrophobic interactions were observed. Separation of very polar compounds including oligosaccharides, nucleic acid bases and nucleosides using Click TE-RA in HILIC mode was successfully accomplished. In addition, separation of saponins both in HILIC and reversed-phase liquid chromatography (RPLC) modes was performed, demonstrating the presence of orthogonality between two different modes on Click TE-RA column. The multiple interactions induced by polar sugar group and hydrophobic aglycone group allowed this Click TE-RA to serve as a multi-mode stationary phase in two-dimensional liquid chromatography. (C) 2015 Elsevier B.V. All rights reserved.
关键词[WOS]: MIXED-MODE CHROMATOGRAPHY ;  SEPARATION ;  RETENTION ;  SACCHARIDES ;  PERFORMANCE ;  SELECTIVITY ;  SAPONINS ;  BEHAVIOR ;  ACIDS
语种: 英语
WOS记录号: WOS:000351792400013
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/146125
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.E China Univ Sci & Technol, Sch Pharm, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Shanghai 200237, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Nat Med, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Liang, Tu,Fu, Qing,Shen, Aijin,et al. Preparation and chromatographic evaluation of a newly designed steviol glycoside modified-silica stationary phase in hydrophilic interaction liquid chromatography and reversed phase liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2015,1388:110-118.
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