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Detection and Chemical Profiling of Ling-Gui-Zhu-Gan Decoction by Ultra Performance Liquid Chromatography-Hybrid Linear Ion Trap-Orbitrap Mass Spectrometry
Wang, Pei1; Wang, Bo2; Xu, Jingyao1; Sun, Jianbo3; Yan, Qin4; Ji, Bin1; Zhao, Yunli1; Yu, Zhiguo1
Source PublicationJOURNAL OF CHROMATOGRAPHIC SCIENCE
2015-02-01
DOI10.1093/chromsci/bmu051
Volume53Issue:2Pages:263-273
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordARRAY DETECTION ; PORIA-COCOS ; LICORICE ; CONSTITUENTS ; PLASMA ; RATS ; METABOLITES ; EXTRACT ; ROOTS ; ACID
AbstractLing-Gui-Zhu-Gan decoction (LGZGD), a well-known traditional Chinese medicine (TCM) formula, has been extensively used for the treatment of cardiovascular disease in clinic. However, the chemical constituents in LGZGD had not been investigated so far. In this study, an ultra performance liquid chromatography-hybrid electrospray ionization linear ion trap-Orbitrap mass spectrometry (UPLC-LTQ-Oribitrap-MS/MS) method was established for rapid separation and structural identification of the constituents in LGZGD. Separation was performed on an ACQUITY (TM) UPLC BEH C-18 column (50 x 2.1 mm, 1.7 mu m) by gradient elution mode, using acetonitrile-water containing 0.1% formic acid as mobile phase at the flow rate of 0.2 mL/min. Accurate mass measurement for molecular ions and characteristic fragment ions could represent identification criteria for these compounds. As a result, 95 compounds including triterpene acids, triterpene saponins, flavonoids, coumarins, coumestans, benzofurans, phenylpropanoids and sesquiterpenoid lactones were detected, and 90 of them were tentatively identified. All compounds were further assigned in the individual raw material. In conclusion, the UPLCLTQ- Orbitrap-MS/MS is a highly efficient technique to separate and identify constituents in complex matrices of TCMs. These results obtained in this research will provide a basis for quality control and further in vivo study of LGZGD.
Language英语
WOS IDWOS:000352281800009
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/146136
Collection中国科学院大连化学物理研究所
Affiliation1.Shenyang Pharmaceut Univ, Sch Pharm, Shenyang 110016, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab High Resolut Mass Spectrometry, Dalian 116023, Peoples R China
3.China Pharmaceut Univ, Dept Nat Med Chem, Nanjing 210009, Peoples R China
4.Laizhou Tradit Chinese Med Hosp, Dept Oncol, Laizhou 261400, Peoples R China
Recommended Citation
GB/T 7714
Wang, Pei,Wang, Bo,Xu, Jingyao,et al. Detection and Chemical Profiling of Ling-Gui-Zhu-Gan Decoction by Ultra Performance Liquid Chromatography-Hybrid Linear Ion Trap-Orbitrap Mass Spectrometry[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2015,53(2):263-273.
APA Wang, Pei.,Wang, Bo.,Xu, Jingyao.,Sun, Jianbo.,Yan, Qin.,...&Yu, Zhiguo.(2015).Detection and Chemical Profiling of Ling-Gui-Zhu-Gan Decoction by Ultra Performance Liquid Chromatography-Hybrid Linear Ion Trap-Orbitrap Mass Spectrometry.JOURNAL OF CHROMATOGRAPHIC SCIENCE,53(2),263-273.
MLA Wang, Pei,et al."Detection and Chemical Profiling of Ling-Gui-Zhu-Gan Decoction by Ultra Performance Liquid Chromatography-Hybrid Linear Ion Trap-Orbitrap Mass Spectrometry".JOURNAL OF CHROMATOGRAPHIC SCIENCE 53.2(2015):263-273.
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