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题名: Detection and Chemical Profiling of Ling-Gui-Zhu-Gan Decoction by Ultra Performance Liquid Chromatography-Hybrid Linear Ion Trap-Orbitrap Mass Spectrometry
作者: Wang, Pei1;  Wang, Bo2;  Xu, Jingyao1;  Sun, Jianbo3;  Yan, Qin4;  Ji, Bin1;  Zhao, Yunli1;  Yu, Zhiguo1
刊名: JOURNAL OF CHROMATOGRAPHIC SCIENCE
发表日期: 2015-02-01
DOI: 10.1093/chromsci/bmu051
卷: 53, 期:2, 页:263-273
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Ling-Gui-Zhu-Gan decoction (LGZGD), a well-known traditional Chinese medicine (TCM) formula, has been extensively used for the treatment of cardiovascular disease in clinic. However, the chemical constituents in LGZGD had not been investigated so far. In this study, an ultra performance liquid chromatography-hybrid electrospray ionization linear ion trap-Orbitrap mass spectrometry (UPLC-LTQ-Oribitrap-MS/MS) method was established for rapid separation and structural identification of the constituents in LGZGD. Separation was performed on an ACQUITY (TM) UPLC BEH C-18 column (50 x 2.1 mm, 1.7 mu m) by gradient elution mode, using acetonitrile-water containing 0.1% formic acid as mobile phase at the flow rate of 0.2 mL/min. Accurate mass measurement for molecular ions and characteristic fragment ions could represent identification criteria for these compounds. As a result, 95 compounds including triterpene acids, triterpene saponins, flavonoids, coumarins, coumestans, benzofurans, phenylpropanoids and sesquiterpenoid lactones were detected, and 90 of them were tentatively identified. All compounds were further assigned in the individual raw material. In conclusion, the UPLCLTQ- Orbitrap-MS/MS is a highly efficient technique to separate and identify constituents in complex matrices of TCMs. These results obtained in this research will provide a basis for quality control and further in vivo study of LGZGD.
关键词[WOS]: ARRAY DETECTION ;  PORIA-COCOS ;  LICORICE ;  CONSTITUENTS ;  PLASMA ;  RATS ;  METABOLITES ;  EXTRACT ;  ROOTS ;  ACID
语种: 英语
WOS记录号: WOS:000352281800009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/146136
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Shenyang Pharmaceut Univ, Sch Pharm, Shenyang 110016, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab High Resolut Mass Spectrometry, Dalian 116023, Peoples R China
3.China Pharmaceut Univ, Dept Nat Med Chem, Nanjing 210009, Peoples R China
4.Laizhou Tradit Chinese Med Hosp, Dept Oncol, Laizhou 261400, Peoples R China

Recommended Citation:
Wang, Pei,Wang, Bo,Xu, Jingyao,et al. Detection and Chemical Profiling of Ling-Gui-Zhu-Gan Decoction by Ultra Performance Liquid Chromatography-Hybrid Linear Ion Trap-Orbitrap Mass Spectrometry[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2015,53(2):263-273.
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