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题名: Insights into the molecular recognition of the granuphilin C2A domain with PI(4,5)P2
作者: Wan, Chanjuan1;  Wu, Bo1;  Song, Zhenwei2, 3;  Zhang, Jiahai2, 3;  Chu, Huiying4;  Wang, Aoli1;  Liu, Qingsong1;  Shi, Yunyu2, 3;  Li, Guohui4;  Wang, Junfeng1
关键词: NMR ;  Granuphilin ;  C2 domain ;  PI(4,5)P2 ;  Nanodisc ;  Recognition mechanism
刊名: CHEMISTRY AND PHYSICS OF LIPIDS
发表日期: 2015-02-01
DOI: 10.1016/j.chemphyslip.2015.01.003
卷: 186, 页:61-67
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ;  Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biophysics
英文摘要: Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) is a key player in regulating the process of excytosis, including insulin secretion. Granuphilin, a tandem C2 domain containing protein, mediates the docking of insulin granules onto plasma membrane. The C2A domain plays key roles in this process through interaction with PI(4,5)P2. In this study, we have investigated the molecular recognition mechanism of granuphilin-C2A domain to PI(4,5)P2 head group, and further to PI(4,5)P2-nanodisc by NMR, ITC, MST and SEC methods. Our results demonstrate that PI(4,5)P2 binds to the concave surface of granuphilin-C2A domain. The key residues involved in the binding were validated by mutation analysis. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
关键词[WOS]: EXOCYTOSIS ;  GRANULES ;  COMPLEX
语种: 英语
WOS记录号: WOS:000352051200008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/146155
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Hefei Inst Phys Sci, High Field Magnet Lab, Hefei 230031, Peoples R China
2.Univ Sci & Technol China, Heifei Natl Lab Phys Sci Microscale, Hefei 230027, Peoples R China
3.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Lab Mol Modeling & Design, Dalian 116023, Peoples R China

Recommended Citation:
Wan, Chanjuan,Wu, Bo,Song, Zhenwei,et al. Insights into the molecular recognition of the granuphilin C2A domain with PI(4,5)P2[J]. CHEMISTRY AND PHYSICS OF LIPIDS,2015,186:61-67.
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