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Insights into the molecular recognition of the granuphilin C2A domain with PI(4,5)P2
Wan, Chanjuan1; Wu, Bo1; Song, Zhenwei2,3; Zhang, Jiahai2,3; Chu, Huiying4; Wang, Aoli1; Liu, Qingsong1; Shi, Yunyu2,3; Li, Guohui4; Wang, Junfeng1
关键词Nmr Granuphilin C2 Domain Nanodisc Pi(4 Recognition Mechanism 5)P2
刊名CHEMISTRY AND PHYSICS OF LIPIDS
2015-02-01
DOI10.1016/j.chemphyslip.2015.01.003
186页:61-67
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]EXOCYTOSIS ; GRANULES ; COMPLEX
英文摘要Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) is a key player in regulating the process of excytosis, including insulin secretion. Granuphilin, a tandem C2 domain containing protein, mediates the docking of insulin granules onto plasma membrane. The C2A domain plays key roles in this process through interaction with PI(4,5)P2. In this study, we have investigated the molecular recognition mechanism of granuphilin-C2A domain to PI(4,5)P2 head group, and further to PI(4,5)P2-nanodisc by NMR, ITC, MST and SEC methods. Our results demonstrate that PI(4,5)P2 binds to the concave surface of granuphilin-C2A domain. The key residues involved in the binding were validated by mutation analysis. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
语种英语
WOS记录号WOS:000352051200008
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/146155
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Hefei Inst Phys Sci, High Field Magnet Lab, Hefei 230031, Peoples R China
2.Univ Sci & Technol China, Heifei Natl Lab Phys Sci Microscale, Hefei 230027, Peoples R China
3.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Lab Mol Modeling & Design, Dalian 116023, Peoples R China
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Wan, Chanjuan,Wu, Bo,Song, Zhenwei,et al. Insights into the molecular recognition of the granuphilin C2A domain with PI(4,5)P2[J]. CHEMISTRY AND PHYSICS OF LIPIDS,2015,186:61-67.
APA Wan, Chanjuan.,Wu, Bo.,Song, Zhenwei.,Zhang, Jiahai.,Chu, Huiying.,...&Wang, Junfeng.(2015).Insights into the molecular recognition of the granuphilin C2A domain with PI(4,5)P2.CHEMISTRY AND PHYSICS OF LIPIDS,186,61-67.
MLA Wan, Chanjuan,et al."Insights into the molecular recognition of the granuphilin C2A domain with PI(4,5)P2".CHEMISTRY AND PHYSICS OF LIPIDS 186(2015):61-67.
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