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Singlet Oxygen Phosphorescence Lifetime Imaging Based on a Fluorescence Lifetime Imaging Microscope
Tian, Wenming1; Deng, Liezheng1; Jin, Shengye1; Yang, Heping1; Cui, Rongrong1; Zhang, Qing2; Shi, Wenbo1; Zhang, Chunlei2; Yuan, Xiaolin2; Sha, Guohe1
刊名JOURNAL OF PHYSICAL CHEMISTRY A
2015-04-09
DOI10.1021/acs.jpca.5b01504
119期:14页:3393-3399
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Physical ; Physics, Atomic, Molecular & Chemical
研究领域[WOS]Chemistry ; Physics
关键词[WOS]OPTICAL-DETECTION ; CELL ; PHOTOLUMINESCENCE ; DIFFUSION ; RESOLUTION ; POLYMERS ; IMAGES ; C-60 ; C60
英文摘要The feasibility of singlet oxygen phosphorescence (SOP) lifetime imaging microscope was studied on a modified fluorescence lifetime imaging microscope (FLIM). SOP results from the infrared radiative transition of O-2(a(1)Delta(g) -> X-3 Sigma(-)(g)) and O-2(a(1)Delta(g)) was produced in a C-60 powder sample via photosensitization process. To capture the very weak SOP signal, a dichroic mirror was placed between the objective and tube lens of the FLIM and used to divide the luminescence returning from the sample into two beams: the reflected SOP beam and the transmitted photoluminescence of C-60 (C-60-PL) beam. The C-60-PL beam entered the scanner of the FLIM and followed the normal optical path of the FLIM, while the SOP steered clear, of the scanner and directly entered a finely designed SOP detection channel Confocal C-60-PL images and nonconfocal SOP images were then simultaneously obtained by using laser scanning mode. Experimental results show that (1) under laser scanning mode, the obstacle to confocal SOP imaging is the infrared incompatible scanner, which can be solved by using an infrared compatible scanner. Confocal SOP imaging is also expected to be realized under stage scanning mode when the laser beam is parked and meanwhile a pinhole is added into the SOP detection channel. (2) A great challenge to SOP imaging is its extraordinarily long imaging time, and selecting only a few interesting points from fluorescence images to measure their SOP time-dependent traces may be a correct compromise.
语种英语
WOS记录号WOS:000352823100010
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/146164
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Dalian 116023, Liaoning, Peoples R China
2.Dalian Univ, Affiliated Zhongshan Hosp, Res Ctr, Dalian 116001, Liaoning, Peoples R China
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GB/T 7714
Tian, Wenming,Deng, Liezheng,Jin, Shengye,et al. Singlet Oxygen Phosphorescence Lifetime Imaging Based on a Fluorescence Lifetime Imaging Microscope[J]. JOURNAL OF PHYSICAL CHEMISTRY A,2015,119(14):3393-3399.
APA Tian, Wenming.,Deng, Liezheng.,Jin, Shengye.,Yang, Heping.,Cui, Rongrong.,...&Sha, Guohe.(2015).Singlet Oxygen Phosphorescence Lifetime Imaging Based on a Fluorescence Lifetime Imaging Microscope.JOURNAL OF PHYSICAL CHEMISTRY A,119(14),3393-3399.
MLA Tian, Wenming,et al."Singlet Oxygen Phosphorescence Lifetime Imaging Based on a Fluorescence Lifetime Imaging Microscope".JOURNAL OF PHYSICAL CHEMISTRY A 119.14(2015):3393-3399.
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