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题名: Singlet Oxygen Phosphorescence Lifetime Imaging Based on a Fluorescence Lifetime Imaging Microscope
作者: Tian, Wenming1;  Deng, Liezheng1;  Jin, Shengye1;  Yang, Heping1;  Cui, Rongrong1;  Zhang, Qing2;  Shi, Wenbo1;  Zhang, Chunlei2;  Yuan, Xiaolin2;  Sha, Guohe1
刊名: JOURNAL OF PHYSICAL CHEMISTRY A
发表日期: 2015-04-09
DOI: 10.1021/acs.jpca.5b01504
卷: 119, 期:14, 页:3393-3399
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Physical ;  Physics, Atomic, Molecular & Chemical
研究领域[WOS]: Chemistry ;  Physics
英文摘要: The feasibility of singlet oxygen phosphorescence (SOP) lifetime imaging microscope was studied on a modified fluorescence lifetime imaging microscope (FLIM). SOP results from the infrared radiative transition of O-2(a(1)Delta(g) -> X-3 Sigma(-)(g)) and O-2(a(1)Delta(g)) was produced in a C-60 powder sample via photosensitization process. To capture the very weak SOP signal, a dichroic mirror was placed between the objective and tube lens of the FLIM and used to divide the luminescence returning from the sample into two beams: the reflected SOP beam and the transmitted photoluminescence of C-60 (C-60-PL) beam. The C-60-PL beam entered the scanner of the FLIM and followed the normal optical path of the FLIM, while the SOP steered clear, of the scanner and directly entered a finely designed SOP detection channel Confocal C-60-PL images and nonconfocal SOP images were then simultaneously obtained by using laser scanning mode. Experimental results show that (1) under laser scanning mode, the obstacle to confocal SOP imaging is the infrared incompatible scanner, which can be solved by using an infrared compatible scanner. Confocal SOP imaging is also expected to be realized under stage scanning mode when the laser beam is parked and meanwhile a pinhole is added into the SOP detection channel. (2) A great challenge to SOP imaging is its extraordinarily long imaging time, and selecting only a few interesting points from fluorescence images to measure their SOP time-dependent traces may be a correct compromise.
关键词[WOS]: OPTICAL-DETECTION ;  CELL ;  PHOTOLUMINESCENCE ;  DIFFUSION ;  RESOLUTION ;  POLYMERS ;  IMAGES ;  C-60 ;  C60
语种: 英语
WOS记录号: WOS:000352823100010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/146164
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Dalian 116023, Liaoning, Peoples R China
2.Dalian Univ, Affiliated Zhongshan Hosp, Res Ctr, Dalian 116001, Liaoning, Peoples R China

Recommended Citation:
Tian, Wenming,Deng, Liezheng,Jin, Shengye,et al. Singlet Oxygen Phosphorescence Lifetime Imaging Based on a Fluorescence Lifetime Imaging Microscope[J]. JOURNAL OF PHYSICAL CHEMISTRY A,2015,119(14):3393-3399.
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