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题名: Characterization of a new endo-type alginate lyase from Vibrio sp W13
作者: Zhu, Benwei1, 2;  Tan, Haidong1;  Qin, Yuqi3;  Xu, Qingsong1;  Du, Yuguang1, 4;  Yin, Heng1
关键词: Alginate lyase ;  Polysaccharide lyase family 7 ;  Vibrio sp.
刊名: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
发表日期: 2015-04-01
DOI: 10.1016/j.ijbiomac.2015.01.053
卷: 75, 页:330-337
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: A gene, encoding a new alginate lyase Algb, was identified and cloned from marine bacterium Vibrio sp. W13. The recombinant alginate lyase was characterized followed by being purified on Ni-NTA Sepharose. It exhibited the highest activity (457 U/mg) at pH 8.0 and 30 degrees C Interestingly, Algb possessed broader substrate specificity. It showed activities toward both polyM (poly beta-D-mannuronate) and polyG (poly alpha-L-guluronate). Furthermore, K-m values of Algb toward alginate (0.67 mg/ml) and polyMG (0.50 mg/ml) are lower than those toward polyG (1.04 mg/ml) and polyM (6.90 mg/ml). The TLC and ESI-MS analysis suggested that Algb mainly released oligosaccharides with DP of 2-5 from the four kinds of substrates in an endolytic manner. Therefore, it may be a potent tool to produce alginate oligosaccharides with low DP. (C) 2015 Elsevier B.V. All rights reserved.
关键词[WOS]: OLIGOALGINATE LYASE ;  SEQUENCE-ANALYSIS ;  SACCHAROPHAGUS DEGRADANS ;  CYSTIC-FIBROSIS ;  RAW264.7 CELLS ;  CLONING ;  PURIFICATION ;  OLIGOSACCHARIDES ;  POLYSACCHARIDE ;  DEPOLYMERIZATION
语种: 英语
WOS记录号: WOS:000352332700044
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/146169
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Shandong Univ, Natl Glycoengn Res Ctr, Jinan 250100, Peoples R China
4.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China

Recommended Citation:
Zhu, Benwei,Tan, Haidong,Qin, Yuqi,et al. Characterization of a new endo-type alginate lyase from Vibrio sp W13[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2015,75:330-337.
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