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题名: An electrochemical deoxyribonucleic acid biosensor for rapid genotoxicity screening of chemicals
作者: Wu, Lidong2;  Lu, Xianbo1;  Wang, Xue1;  Song, Yi2;  Chen, Jiping1
刊名: ANALYTICAL METHODS
发表日期: 2015
DOI: 10.1039/c5ay00020c
卷: 7, 期:8, 页:3347-3352
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences ;  Life Sciences & Biomedicine ;  Technology
类目[WOS]: Chemistry, Analytical ;  Food Science & Technology ;  Spectroscopy
研究领域[WOS]: Chemistry ;  Food Science & Technology ;  Spectroscopy
英文摘要: A sensitive electrochemical biosensor based on double-stranded deoxyribonucleic acid (DNA) has been proposed for rapid screening of chemicals genotoxicity potential. A DNA probe from the clone RP3-402G11 gene of the human DNA sequence and electroactive methylene blue (MB) have been used as a biorecognition element and signal amplification molecules respectively for evaluating the genotoxic potential of target analytes with high sensitivity. The biosensing mechanism of genotoxicity screening is based on the damage of targets for the DNA double helix, which results in the subsequent distinct change of the electrochemical signal. More than 10 kinds of genotoxic chemicals have been used as testing analytes including highly toxic dioxins (polychlorinated dibenzodioxins, polychlorinated dibenzofurans) and polychlorinated biphenyls (PCBs). Dioxins and dioxin-like chemicals have been identified as highly genotoxic chemicals by the proposed DNA biosensor, which is consistent with the conclusion from International Agency for Research on Cancer. The results obtained demonstrated that the signal response of the biosensor for dioxins and PCBs correlated well with their toxic equivalent factor (TEF) values and concentrations of tested targets. The biosensor proved to be a promising in vitro screening tool for rapid estimation of chemicals genotoxicity potential.
关键词[WOS]: DNA-MODIFIED ELECTRODE ;  WASTE-WATER SAMPLES ;  DIBENZO-P-DIOXINS ;  METHYLENE-BLUE ;  HYBRIDIZATION INDICATOR ;  GOLD ELECTRODES ;  TOXICITY ;  PCBS ;  COMBUSTION ;  PRODUCTS
语种: 英语
WOS记录号: WOS:000352897500006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/146183
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Fishery Sci, Beijing 100141, Peoples R China

Recommended Citation:
Wu, Lidong,Lu, Xianbo,Wang, Xue,et al. An electrochemical deoxyribonucleic acid biosensor for rapid genotoxicity screening of chemicals[J]. ANALYTICAL METHODS,2015,7(8):3347-3352.
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