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The influence of organic sample solvents on the separation efficiency of basic compounds under strong cation exchange mode
Long, Zhen1; Yu, Dongping1; Liu, Yanfang1; Du, Nana1; Tao, Yanduo2; Mei, Lijuan2; Guo, Zhimou1; Liang, Xinmiao1
KeywordStrong Cation Exchange Separation Efficiency Solvent Elution Strength Solvent Injection Volume
Source PublicationANALYTICA CHIMICA ACTA
2015-05-04
DOI10.1016/j.aca.2014.12.057
Volume872Pages:77-83
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; REVERSED STATIONARY PHASES ; INJECTION VOLUME ; MOBILE-PHASE ; PEAK SHAPE ; DRUGS ; BERBERINE ; HPLC ; IDENTIFICATION ; PURIFICATION
AbstractThis study investigated the influence of organic sample solvents on separation efficiency of basic compounds under strong cation exchange (SCX) mode. The mixtures of acidic aqueous solution and organic solvent such as acetonitrile, ethanol, methanol and dimethyl sulfoxide (DMSO) were tested as sample solvents. For later-eluting analytes, the increase of sample solvent elution strength was responsible for the decrease of separation efficiency. Thus, sample solvents with weak elution strength could provide high separation efficiencies. For earlier-eluting analytes, the retention of organic sample solvents was the main factor affecting separation efficiency. Weakly retained solvents could provide high separation efficiency. In addition, an optimized approach was proposed to reduce the effect of organic sample solvent, in which low ionic solvent was employed as initial mobile phase in the gradient. At last, the analysis of impurities in hydrophobic drug berberine was performed. The results showed that using acidic aqueous methanol as sample solvents could provide high separation efficiency and good resolution (R > 1.5). (C) 2015 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000353218600009
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/146184
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Nat Med, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810008, Peoples R China
Recommended Citation
GB/T 7714
Long, Zhen,Yu, Dongping,Liu, Yanfang,et al. The influence of organic sample solvents on the separation efficiency of basic compounds under strong cation exchange mode[J]. ANALYTICA CHIMICA ACTA,2015,872:77-83.
APA Long, Zhen.,Yu, Dongping.,Liu, Yanfang.,Du, Nana.,Tao, Yanduo.,...&Liang, Xinmiao.(2015).The influence of organic sample solvents on the separation efficiency of basic compounds under strong cation exchange mode.ANALYTICA CHIMICA ACTA,872,77-83.
MLA Long, Zhen,et al."The influence of organic sample solvents on the separation efficiency of basic compounds under strong cation exchange mode".ANALYTICA CHIMICA ACTA 872(2015):77-83.
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