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题名: Evaluation of a silicon oxynitride hydrophilic interaction liquid chromatography column in saccharide and glycoside separations
作者: Wan, Huihui1;  Sheng, Qianying2;  Zhong, Hongmin1;  Guo, Xiujie3;  Fu, Qing2;  Liu, Yanfang3;  Xue, Xingya3;  Liang, Xinmiao3
关键词: Carbohydrates ;  Hydrophilic interaction liquid chromatography ;  Silica ;  Silicon oxynitride ;  Stationary phases
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2015-04-01
DOI: 10.1002/jssc.201401413
卷: 38, 期:8, 页:1271-1276
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: The retention characteristics of a silicon oxynitride stationary phase for carbohydrate separation were studied in hydrophilic interaction chromatography mode. Four saccharides including mono-, di-, and trisaccharides were employed to investigate the effects of water content and buffer concentration in the mobile phase on hydrophilic interaction liquid chromatography retention. For the tested saccharides, the silicon oxynitride column demonstrated excellent performance in terms of separation efficiency, hydrophilicity, and interesting separation selectivity for carbohydrates compared to the bare silica stationary phase. Finally, the silicon oxynitride hydrophilic interaction liquid chromatography column was employed in the separation of complex samples of fructooligosaccharides, saponins, and steviol glycoside from natural products. The resulting chromatograms demonstrated good separation efficiency and longer retention compared with silica, which further confirmed the advantages and potential application of silicon oxynitride stationary phase for hydrophilic interaction liquid chromatography separation.
关键词[WOS]: ANION-EXCHANGE CHROMATOGRAPHY ;  STATIONARY PHASES ;  MASS-SPECTROMETRY ;  POLAR COMPOUNDS ;  PERFORMANCE ;  SELECTIVITY ;  RETENTION ;  SURFACE ;  METABOLOMICS ;  PEPTIDES
语种: 英语
WOS记录号: WOS:000353488200002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/146189
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Univ Technol, Analyt Ctr, Fac Chem Environm & Biol Sci & Technol, Dalian 116024, Peoples R China
2.E China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Sch Pharm, Shanghai 200237, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China

Recommended Citation:
Wan, Huihui,Sheng, Qianying,Zhong, Hongmin,et al. Evaluation of a silicon oxynitride hydrophilic interaction liquid chromatography column in saccharide and glycoside separations[J]. JOURNAL OF SEPARATION SCIENCE,2015,38(8):1271-1276.
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