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Evaluation of a silicon oxynitride hydrophilic interaction liquid chromatography column in saccharide and glycoside separations
Wan, Huihui1; Sheng, Qianying2; Zhong, Hongmin1; Guo, Xiujie3; Fu, Qing2; Liu, Yanfang3; Xue, Xingya3; Liang, Xinmiao3
关键词Carbohydrates Hydrophilic Interaction Liquid Chromatography Silica Silicon Oxynitride Stationary Phases
刊名JOURNAL OF SEPARATION SCIENCE
2015-04-01
DOI10.1002/jssc.201401413
38期:8页:1271-1276
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]ANION-EXCHANGE CHROMATOGRAPHY ; STATIONARY PHASES ; MASS-SPECTROMETRY ; POLAR COMPOUNDS ; PERFORMANCE ; SELECTIVITY ; RETENTION ; SURFACE ; METABOLOMICS ; PEPTIDES
英文摘要The retention characteristics of a silicon oxynitride stationary phase for carbohydrate separation were studied in hydrophilic interaction chromatography mode. Four saccharides including mono-, di-, and trisaccharides were employed to investigate the effects of water content and buffer concentration in the mobile phase on hydrophilic interaction liquid chromatography retention. For the tested saccharides, the silicon oxynitride column demonstrated excellent performance in terms of separation efficiency, hydrophilicity, and interesting separation selectivity for carbohydrates compared to the bare silica stationary phase. Finally, the silicon oxynitride hydrophilic interaction liquid chromatography column was employed in the separation of complex samples of fructooligosaccharides, saponins, and steviol glycoside from natural products. The resulting chromatograms demonstrated good separation efficiency and longer retention compared with silica, which further confirmed the advantages and potential application of silicon oxynitride stationary phase for hydrophilic interaction liquid chromatography separation.
语种英语
WOS记录号WOS:000353488200002
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/146189
专题中国科学院大连化学物理研究所
作者单位1.Dalian Univ Technol, Analyt Ctr, Fac Chem Environm & Biol Sci & Technol, Dalian 116024, Peoples R China
2.E China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Sch Pharm, Shanghai 200237, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
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GB/T 7714
Wan, Huihui,Sheng, Qianying,Zhong, Hongmin,et al. Evaluation of a silicon oxynitride hydrophilic interaction liquid chromatography column in saccharide and glycoside separations[J]. JOURNAL OF SEPARATION SCIENCE,2015,38(8):1271-1276.
APA Wan, Huihui.,Sheng, Qianying.,Zhong, Hongmin.,Guo, Xiujie.,Fu, Qing.,...&Liang, Xinmiao.(2015).Evaluation of a silicon oxynitride hydrophilic interaction liquid chromatography column in saccharide and glycoside separations.JOURNAL OF SEPARATION SCIENCE,38(8),1271-1276.
MLA Wan, Huihui,et al."Evaluation of a silicon oxynitride hydrophilic interaction liquid chromatography column in saccharide and glycoside separations".JOURNAL OF SEPARATION SCIENCE 38.8(2015):1271-1276.
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