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Hydrophilic-subtraction model for the characterization and comparison of hydrophilic interaction liquid chromatography columns
Wang, Jixia; Guo, Zhimou; Shen, Aijin; Yu, Long; Xiao, Yuansheng; Xue, Xingya; Zhang, Xiuli; Liang, Xinmiao
KeywordHydrophilic-subtraction Model Hilic Columns Retention Mechanisms Selectivity
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2015-06-12
DOI10.1016/j.chroma.2015.03.065
Volume1398Pages:29-46
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordSOLVATION ENERGY RELATIONSHIPS ; ZWITTERIONIC STATIONARY-PHASE ; RETENTION PREDICTION MODELS ; REVERSED-PHASE ; ADRENORECEPTOR AGONISTS ; MASS-SPECTROMETRY ; POLAR COMPOUNDS ; SILICA COLUMNS ; SEPARATION ; SELECTIVITY
AbstractNowadays more and more hydrophilic interaction liquid chromatography (HILIC) columns with diverse functional groups have become commercially available, which pose a challenge to select an appropriate one. However, there is no universal model to provide guidance for selecting HILIC columns. To handle this problem, a retention model named "hydrophilic-subtraction model" was developed to characterize and compare HILIC columns. The hydrophilic-subtraction model, which was designed based on the widely recognized HILIC retention mechanisms including hydrophilic partitioning, hydrogen-bonding and electrostatic interactions, was established by the retention of 41 solutes with various properties on 8 representative HILIC columns. High correlation coefficients (R-2 >= 0.990) and small standard deviations (SD <= 0.041) indicated that this model correlated effectively the retention with solute descriptors and column parameters. To evaluate reliability of the model, the model was further applied to characterize 15 additional HILIC columns using 41 solutes. The results of multiple linear regression confirmed the significance of the model. The regression coefficients of the model were used to investigate retention mechanisms occurring in different chromatographic systems. Based on these regression coefficients, selectivities of HILIC stationary phases were exhibited intuitively by an angle graph and a spider diagram, which could be used as guidance for researchers to select appropriate columns for HILIC separation. Additionally, a rapid and convenient procedure was proposed for characterizing HILIC columns. (C) 2015 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000355054300004
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/146244
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Wang, Jixia,Guo, Zhimou,Shen, Aijin,et al. Hydrophilic-subtraction model for the characterization and comparison of hydrophilic interaction liquid chromatography columns[J]. JOURNAL OF CHROMATOGRAPHY A,2015,1398:29-46.
APA Wang, Jixia.,Guo, Zhimou.,Shen, Aijin.,Yu, Long.,Xiao, Yuansheng.,...&Liang, Xinmiao.(2015).Hydrophilic-subtraction model for the characterization and comparison of hydrophilic interaction liquid chromatography columns.JOURNAL OF CHROMATOGRAPHY A,1398,29-46.
MLA Wang, Jixia,et al."Hydrophilic-subtraction model for the characterization and comparison of hydrophilic interaction liquid chromatography columns".JOURNAL OF CHROMATOGRAPHY A 1398(2015):29-46.
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