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题名: Hydrophilic-subtraction model for the characterization and comparison of hydrophilic interaction liquid chromatography columns
作者: Wang, Jixia1;  Guo, Zhimou1;  Shen, Aijin1;  Yu, Long1;  Xiao, Yuansheng1;  Xue, Xingya1;  Zhang, Xiuli1;  Liang, Xinmiao1
关键词: Hydrophilic-subtraction model ;  HILIC columns ;  Retention mechanisms ;  Selectivity
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2015-06-12
DOI: 10.1016/j.chroma.2015.03.065
卷: 1398, 页:29-46
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Nowadays more and more hydrophilic interaction liquid chromatography (HILIC) columns with diverse functional groups have become commercially available, which pose a challenge to select an appropriate one. However, there is no universal model to provide guidance for selecting HILIC columns. To handle this problem, a retention model named "hydrophilic-subtraction model" was developed to characterize and compare HILIC columns. The hydrophilic-subtraction model, which was designed based on the widely recognized HILIC retention mechanisms including hydrophilic partitioning, hydrogen-bonding and electrostatic interactions, was established by the retention of 41 solutes with various properties on 8 representative HILIC columns. High correlation coefficients (R-2 >= 0.990) and small standard deviations (SD <= 0.041) indicated that this model correlated effectively the retention with solute descriptors and column parameters. To evaluate reliability of the model, the model was further applied to characterize 15 additional HILIC columns using 41 solutes. The results of multiple linear regression confirmed the significance of the model. The regression coefficients of the model were used to investigate retention mechanisms occurring in different chromatographic systems. Based on these regression coefficients, selectivities of HILIC stationary phases were exhibited intuitively by an angle graph and a spider diagram, which could be used as guidance for researchers to select appropriate columns for HILIC separation. Additionally, a rapid and convenient procedure was proposed for characterizing HILIC columns. (C) 2015 Elsevier B.V. All rights reserved.
关键词[WOS]: SOLVATION ENERGY RELATIONSHIPS ;  ZWITTERIONIC STATIONARY-PHASE ;  RETENTION PREDICTION MODELS ;  REVERSED-PHASE ;  ADRENORECEPTOR AGONISTS ;  MASS-SPECTROMETRY ;  POLAR COMPOUNDS ;  SILICA COLUMNS ;  SEPARATION ;  SELECTIVITY
语种: 英语
WOS记录号: WOS:000355054300004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/146244
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Wang, Jixia,Guo, Zhimou,Shen, Aijin,et al. Hydrophilic-subtraction model for the characterization and comparison of hydrophilic interaction liquid chromatography columns[J]. JOURNAL OF CHROMATOGRAPHY A,2015,1398:29-46.
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