DICP OpenIR
Directly probing redox-linked quinones in photosystem II membrane fragments via UV resonance Raman scattering
Chen, Jun1,2; Yao, Mingdong1; Pagba, Cynthia V.3,4; Zheng, Yang1; Fei, Liping1; Feng, Zhaochi2; Barry, Bridgette A.3,4
关键词Carbonyl Bond Photosynthesis Proton Transfer Electron Transfer Lipid Membrane Protein Dynamics
刊名BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
2015-06-01
DOI10.1016/j.bbabio.2015.03.002
1847期:6-7页:558-564
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]TRANSFORM INFRARED-SPECTROSCOPY ; ELECTRON-TRANSFER ; REACTION CENTERS ; SPECTRA ; ACCEPTOR ; 1,4-BENZOQUINONE ; REDUCTION ; DYNAMICS ; BINDING ; Q(A)
英文摘要In photosynthesis, photosystem II (PSII) harvests sunlight with bound pigments to oxidize water and reduce quinone to quinol, which serves as electron and proton mediators for solar-to-chemical energy conversion. At least two types of quinone cofactors in PSII are redox-linked: Q(A), and Q(B). Here, we for the first time apply 257-nm ultraviolet resonance Raman (UVRR) spectroscopy to acquire the molecular vibrations of plastoquinone (PQ) in PSII membranes. Owing to the resonance enhancement effect, the vibrational signal of PQ in PSII membranes is prominent. A strong band at 1661 cm(-1) is assigned to ring C=C/C=0 symmetric stretch mode (nu 8a mode) of PQ and a weak band at 469 cm(-1) to ring stretch mode. By using a pump-probe difference UVRR method and a sample jet technique, the signals of QA and QB can be distinguished. A frequency difference of 1.4 cm(-1) in nu 8a vibrational mode between QA and QB is observed, corresponding to similar to 86 mV redox potential difference imposed by their protein environment. In addition, there are other PQs in the PSII membranes. A negligible anharmonicity effect on their combination band at 2130 cm(-1) suggests that the 'other PQs' are situated in a hydrophobic environment. The detection of the 'other PQs' might be consistent with the view that another functional PQ cofactor (not Q(A) or Q(B)) exists in PSII. This UVRR approach will be useful to the study of quinone molecules in photosynthesis or other biological systems. (C) 2015 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000355054900007
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/146246
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian Natl Lab Clean Energy, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China
3.Georgia Inst Technol, Sch Chem & Biochem, Atlanta, GA 30332 USA
4.Georgia Inst Technol, Petit Inst Bioengn & Biosci, Atlanta, GA 30332 USA
推荐引用方式
GB/T 7714
Chen, Jun,Yao, Mingdong,Pagba, Cynthia V.,et al. Directly probing redox-linked quinones in photosystem II membrane fragments via UV resonance Raman scattering[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,2015,1847(6-7):558-564.
APA Chen, Jun.,Yao, Mingdong.,Pagba, Cynthia V..,Zheng, Yang.,Fei, Liping.,...&Barry, Bridgette A..(2015).Directly probing redox-linked quinones in photosystem II membrane fragments via UV resonance Raman scattering.BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,1847(6-7),558-564.
MLA Chen, Jun,et al."Directly probing redox-linked quinones in photosystem II membrane fragments via UV resonance Raman scattering".BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS 1847.6-7(2015):558-564.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Chen, Jun]的文章
[Yao, Mingdong]的文章
[Pagba, Cynthia V.]的文章
百度学术
百度学术中相似的文章
[Chen, Jun]的文章
[Yao, Mingdong]的文章
[Pagba, Cynthia V.]的文章
必应学术
必应学术中相似的文章
[Chen, Jun]的文章
[Yao, Mingdong]的文章
[Pagba, Cynthia V.]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。