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题名: Directly probing redox-linked quinones in photosystem II membrane fragments via UV resonance Raman scattering
作者: Chen, Jun1, 2;  Yao, Mingdong1;  Pagba, Cynthia V.3, 4;  Zheng, Yang1;  Fei, Liping1;  Feng, Zhaochi2;  Barry, Bridgette A.3, 4
关键词: Carbonyl bond ;  Photosynthesis ;  Proton transfer ;  Electron transfer ;  Lipid membrane ;  Protein dynamics
刊名: BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
发表日期: 2015-06-01
DOI: 10.1016/j.bbabio.2015.03.002
卷: 1847, 期:6-7, 页:558-564
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ;  Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biophysics
英文摘要: In photosynthesis, photosystem II (PSII) harvests sunlight with bound pigments to oxidize water and reduce quinone to quinol, which serves as electron and proton mediators for solar-to-chemical energy conversion. At least two types of quinone cofactors in PSII are redox-linked: Q(A), and Q(B). Here, we for the first time apply 257-nm ultraviolet resonance Raman (UVRR) spectroscopy to acquire the molecular vibrations of plastoquinone (PQ) in PSII membranes. Owing to the resonance enhancement effect, the vibrational signal of PQ in PSII membranes is prominent. A strong band at 1661 cm(-1) is assigned to ring C=C/C=0 symmetric stretch mode (nu 8a mode) of PQ and a weak band at 469 cm(-1) to ring stretch mode. By using a pump-probe difference UVRR method and a sample jet technique, the signals of QA and QB can be distinguished. A frequency difference of 1.4 cm(-1) in nu 8a vibrational mode between QA and QB is observed, corresponding to similar to 86 mV redox potential difference imposed by their protein environment. In addition, there are other PQs in the PSII membranes. A negligible anharmonicity effect on their combination band at 2130 cm(-1) suggests that the 'other PQs' are situated in a hydrophobic environment. The detection of the 'other PQs' might be consistent with the view that another functional PQ cofactor (not Q(A) or Q(B)) exists in PSII. This UVRR approach will be useful to the study of quinone molecules in photosynthesis or other biological systems. (C) 2015 Elsevier B.V. All rights reserved.
关键词[WOS]: TRANSFORM INFRARED-SPECTROSCOPY ;  ELECTRON-TRANSFER ;  REACTION CENTERS ;  SPECTRA ;  ACCEPTOR ;  1,4-BENZOQUINONE ;  REDUCTION ;  DYNAMICS ;  BINDING ;  Q(A)
语种: 英语
WOS记录号: WOS:000355054900007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/146246
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian Natl Lab Clean Energy, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China
3.Georgia Inst Technol, Sch Chem & Biochem, Atlanta, GA 30332 USA
4.Georgia Inst Technol, Petit Inst Bioengn & Biosci, Atlanta, GA 30332 USA

Recommended Citation:
Chen, Jun,Yao, Mingdong,Pagba, Cynthia V.,et al. Directly probing redox-linked quinones in photosystem II membrane fragments via UV resonance Raman scattering[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,2015,1847(6-7):558-564.
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