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题名: Growth and production of microencapsulated recombinant CHO in a stirred tank bioreactor
作者: Wang, Yu1, 2;  Zhang, Ying1;  Li, Na1, 2;  Chen, Li1, 2;  Zhang, Demeng1, 2;  Sun, Dongsheng1, 2;  Lv, Guojun1;  Yu, Weiting1;  Guo, Xin1;  Ma, Xiaojun1
关键词: Cell culture ;  Stirred tank bioreactor ;  Microencapsulation ;  Recombinant protein production
刊名: BIOPROCESS AND BIOSYSTEMS ENGINEERING
发表日期: 2015-07-01
DOI: 10.1007/s00449-015-1370-2
卷: 38, 期:7, 页:1303-1312
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Technology
类目[WOS]: Biotechnology & Applied Microbiology ;  Engineering, Chemical
研究领域[WOS]: Biotechnology & Applied Microbiology ;  Engineering
英文摘要: Microencapsulation supplies cells with a three-dimensional microenvironment enhancing the metabolic activity, cell density and recombinant protein expression in a stirred tank bioreactor which is used widely to culture mammalian cells in many biochemical processes. In this paper, we address the growth and Desmodus rotundus salivary plasminogen activator (DSPA) production of recombinant CHO (rCHO) in a stirred tank bioreactor. Cells were cultured using two different methods-in an unmicroencapsulated versus microencapsulated culture-and compared differences between them in terms of cell reproduction and DSPA protein productivity. Compared to the unmicroencapsulated rCHO, microencapsulated cells got higher cell density and prolonged the plateau phase. Microencapsulated rCHO promoted DSPA production, with a maximum rate that was 4.8 times higher than in unmicroencapsulated cells, and the accumulated production of DSPA was 3.3 higher than in unmicroencapsulated cells. Negative relationship was found between specific growth rate and DSPA production capacity of unit cells. These findings will facilitate the methods for higher DSPA production in stirred tank bioreactors.
关键词[WOS]: BIOPHARMACEUTICAL BENCHMARKS ;  HEPATOCYTE ENCAPSULATION ;  CELL-CULTURE ;  MICROCAPSULES ;  STABILITY ;  OXYGEN ;  SERUM
语种: 英语
WOS记录号: WOS:000355940900008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/146295
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Wang, Yu,Zhang, Ying,Li, Na,et al. Growth and production of microencapsulated recombinant CHO in a stirred tank bioreactor[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2015,38(7):1303-1312.
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