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Growth and production of microencapsulated recombinant CHO in a stirred tank bioreactor
Wang, Yu1,2; Zhang, Ying1; Li, Na1,2; Chen, Li1,2; Zhang, Demeng1,2; Sun, Dongsheng1,2; Lv, Guojun1; Yu, Weiting1; Guo, Xin1; Ma, Xiaojun1
关键词Cell Culture Stirred Tank Bioreactor Microencapsulation Recombinant Protein Production
刊名BIOPROCESS AND BIOSYSTEMS ENGINEERING
2015-07-01
DOI10.1007/s00449-015-1370-2
38期:7页:1303-1312
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]Biotechnology & Applied Microbiology ; Engineering, Chemical
研究领域[WOS]Biotechnology & Applied Microbiology ; Engineering
关键词[WOS]BIOPHARMACEUTICAL BENCHMARKS ; HEPATOCYTE ENCAPSULATION ; CELL-CULTURE ; MICROCAPSULES ; STABILITY ; OXYGEN ; SERUM
英文摘要Microencapsulation supplies cells with a three-dimensional microenvironment enhancing the metabolic activity, cell density and recombinant protein expression in a stirred tank bioreactor which is used widely to culture mammalian cells in many biochemical processes. In this paper, we address the growth and Desmodus rotundus salivary plasminogen activator (DSPA) production of recombinant CHO (rCHO) in a stirred tank bioreactor. Cells were cultured using two different methods-in an unmicroencapsulated versus microencapsulated culture-and compared differences between them in terms of cell reproduction and DSPA protein productivity. Compared to the unmicroencapsulated rCHO, microencapsulated cells got higher cell density and prolonged the plateau phase. Microencapsulated rCHO promoted DSPA production, with a maximum rate that was 4.8 times higher than in unmicroencapsulated cells, and the accumulated production of DSPA was 3.3 higher than in unmicroencapsulated cells. Negative relationship was found between specific growth rate and DSPA production capacity of unit cells. These findings will facilitate the methods for higher DSPA production in stirred tank bioreactors.
语种英语
WOS记录号WOS:000355940900008
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/146295
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714
Wang, Yu,Zhang, Ying,Li, Na,et al. Growth and production of microencapsulated recombinant CHO in a stirred tank bioreactor[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2015,38(7):1303-1312.
APA Wang, Yu.,Zhang, Ying.,Li, Na.,Chen, Li.,Zhang, Demeng.,...&Ma, Xiaojun.(2015).Growth and production of microencapsulated recombinant CHO in a stirred tank bioreactor.BIOPROCESS AND BIOSYSTEMS ENGINEERING,38(7),1303-1312.
MLA Wang, Yu,et al."Growth and production of microencapsulated recombinant CHO in a stirred tank bioreactor".BIOPROCESS AND BIOSYSTEMS ENGINEERING 38.7(2015):1303-1312.
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