DICP OpenIR
Growth and production of microencapsulated recombinant CHO in a stirred tank bioreactor
Wang, Yu1,2; Zhang, Ying1; Li, Na1,2; Chen, Li1,2; Zhang, Demeng1,2; Sun, Dongsheng1,2; Lv, Guojun1; Yu, Weiting1; Guo, Xin1; Ma, Xiaojun1
KeywordCell Culture Stirred Tank Bioreactor Microencapsulation Recombinant Protein Production
Source PublicationBIOPROCESS AND BIOSYSTEMS ENGINEERING
2015-07-01
DOI10.1007/s00449-015-1370-2
Volume38Issue:7Pages:1303-1312
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS KeywordBIOPHARMACEUTICAL BENCHMARKS ; HEPATOCYTE ENCAPSULATION ; CELL-CULTURE ; MICROCAPSULES ; STABILITY ; OXYGEN ; SERUM
AbstractMicroencapsulation supplies cells with a three-dimensional microenvironment enhancing the metabolic activity, cell density and recombinant protein expression in a stirred tank bioreactor which is used widely to culture mammalian cells in many biochemical processes. In this paper, we address the growth and Desmodus rotundus salivary plasminogen activator (DSPA) production of recombinant CHO (rCHO) in a stirred tank bioreactor. Cells were cultured using two different methods-in an unmicroencapsulated versus microencapsulated culture-and compared differences between them in terms of cell reproduction and DSPA protein productivity. Compared to the unmicroencapsulated rCHO, microencapsulated cells got higher cell density and prolonged the plateau phase. Microencapsulated rCHO promoted DSPA production, with a maximum rate that was 4.8 times higher than in unmicroencapsulated cells, and the accumulated production of DSPA was 3.3 higher than in unmicroencapsulated cells. Negative relationship was found between specific growth rate and DSPA production capacity of unit cells. These findings will facilitate the methods for higher DSPA production in stirred tank bioreactors.
Language英语
WOS IDWOS:000355940900008
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/146295
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Wang, Yu,Zhang, Ying,Li, Na,et al. Growth and production of microencapsulated recombinant CHO in a stirred tank bioreactor[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2015,38(7):1303-1312.
APA Wang, Yu.,Zhang, Ying.,Li, Na.,Chen, Li.,Zhang, Demeng.,...&Ma, Xiaojun.(2015).Growth and production of microencapsulated recombinant CHO in a stirred tank bioreactor.BIOPROCESS AND BIOSYSTEMS ENGINEERING,38(7),1303-1312.
MLA Wang, Yu,et al."Growth and production of microencapsulated recombinant CHO in a stirred tank bioreactor".BIOPROCESS AND BIOSYSTEMS ENGINEERING 38.7(2015):1303-1312.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Yu]'s Articles
[Zhang, Ying]'s Articles
[Li, Na]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Yu]'s Articles
[Zhang, Ying]'s Articles
[Li, Na]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Yu]'s Articles
[Zhang, Ying]'s Articles
[Li, Na]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.