DICP OpenIR
Illuminating biological processes through site-specific protein labeling
Zhang, Gong1,2; Zheng, Siqi3; Liu, Haiping3; Chen, Peng R.2,3
Source PublicationCHEMICAL SOCIETY REVIEWS
2015
DOI10.1039/c4cs00393d
Volume44Issue:11Pages:3405-3417
Indexed BySCI
SubtypeReview
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordUNNATURAL AMINO-ACIDS ; CELL-SURFACE PROTEINS ; GENETIC-CODE ; MAMMALIAN-CELLS ; LIVING CELLS ; CHEMISTRY ; FLUORESCENCE ; ACTIVATION ; MICROSCOPY ; FLUOROPHORE
AbstractCoupling genetically encoded peptide tags or unnatural amino acids (UAAs) with bioorthogonal reactions allows for precise control over the protein-labeling sites as well as the wide choice of labeling dyes. However, the value of these site-specific protein labeling strategies in a real biology setting, particularly their advantages over conventional labeling methods including fluorescent proteins (FPs), remains to be fully demonstrated. In this tutorial review, we first introduce various strategies for site-specific protein labeling that utilize artificial peptide sequences or genetically encoded UAAs as the labeling handle. Emphasis will be placed on introducing the advantages of protein site-specific labeling techniques as well as their applications in solving biological problems, particularly as to why a site-specific protein labeling approach is needed. Finally, beyond the widely used single site-specific labeling methods, the recently emerged dual site-specific protein labeling strategies will be introduced together with their fast-growing potential in illustrating biological processes.
Language英语
WOS IDWOS:000355485900004
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/146303
Collection中国科学院大连化学物理研究所
Affiliation1.Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China
2.Peking Tsinghua Ctr Life Sci, Beijing, Peoples R China
3.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Synthet & Funct Biomol Ctr,Coll Chem, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Gong,Zheng, Siqi,Liu, Haiping,et al. Illuminating biological processes through site-specific protein labeling[J]. CHEMICAL SOCIETY REVIEWS,2015,44(11):3405-3417.
APA Zhang, Gong,Zheng, Siqi,Liu, Haiping,&Chen, Peng R..(2015).Illuminating biological processes through site-specific protein labeling.CHEMICAL SOCIETY REVIEWS,44(11),3405-3417.
MLA Zhang, Gong,et al."Illuminating biological processes through site-specific protein labeling".CHEMICAL SOCIETY REVIEWS 44.11(2015):3405-3417.
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