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题名: Illuminating biological processes through site-specific protein labeling
作者: Zhang, Gong1, 2;  Zheng, Siqi3;  Liu, Haiping3;  Chen, Peng R.2, 3
刊名: CHEMICAL SOCIETY REVIEWS
发表日期: 2015
DOI: 10.1039/c4cs00393d
卷: 44, 期:11, 页:3405-3417
收录类别: SCI
文章类型: Review
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: Coupling genetically encoded peptide tags or unnatural amino acids (UAAs) with bioorthogonal reactions allows for precise control over the protein-labeling sites as well as the wide choice of labeling dyes. However, the value of these site-specific protein labeling strategies in a real biology setting, particularly their advantages over conventional labeling methods including fluorescent proteins (FPs), remains to be fully demonstrated. In this tutorial review, we first introduce various strategies for site-specific protein labeling that utilize artificial peptide sequences or genetically encoded UAAs as the labeling handle. Emphasis will be placed on introducing the advantages of protein site-specific labeling techniques as well as their applications in solving biological problems, particularly as to why a site-specific protein labeling approach is needed. Finally, beyond the widely used single site-specific labeling methods, the recently emerged dual site-specific protein labeling strategies will be introduced together with their fast-growing potential in illustrating biological processes.
关键词[WOS]: UNNATURAL AMINO-ACIDS ;  CELL-SURFACE PROTEINS ;  GENETIC-CODE ;  MAMMALIAN-CELLS ;  LIVING CELLS ;  CHEMISTRY ;  FLUORESCENCE ;  ACTIVATION ;  MICROSCOPY ;  FLUOROPHORE
语种: 英语
WOS记录号: WOS:000355485900004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/146303
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China
2.Peking Tsinghua Ctr Life Sci, Beijing, Peoples R China
3.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Synthet & Funct Biomol Ctr,Coll Chem, Beijing 100871, Peoples R China

Recommended Citation:
Zhang, Gong,Zheng, Siqi,Liu, Haiping,et al. Illuminating biological processes through site-specific protein labeling[J]. CHEMICAL SOCIETY REVIEWS,2015,44(11):3405-3417.
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