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题名: Lipidome and metabolome analysis of fresh tobacco leaves in different geographical regions using liquid chromatography-mass spectrometry
作者: Li, Lili1;  Lu, Xin1;  Zhao, Jieyu1;  Zhang, Junjie1;  Zhao, Yanni1;  Zhao, Chunxia1;  Xu, Guowang1
关键词: Lipidome ;  Metabolome ;  Fresh tobacco leaves ;  Climatic factors ;  Metabolomics
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2015-07-01
DOI: 10.1007/s00216-015-8522-8
卷: 407, 期:17, 页:5009-5020
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: The combination of the lipidome and the metabolome can provide much more information in plant metabolomics studies. A method for the simultaneous extraction of the lipidome and the metabolome of fresh tobacco leaves was developed. Method validation was performed on the basis of the optimal ratio of methanol to methyl tert-butyl ether to water (37:45:68) from the design of experiments. Good repeatability was obtained. We found that 92.2 % and 91.6 % of the peaks for the lipidome and the metabolome were within a relative standard deviation of 20 %, accounting for 94.6 % and 94.6 % of the total abundance, respectively. The intraday and interday precisions were also satisfactory. A total of 230 metabolites, including 129 lipids, were identified. Significant differences were found in lipidomic and metabolomic profiles of fresh tobacco leaves in different geographical regions. Highly unsaturated galactolipids, phosphatidylethanolamines, predominant phosphatidylcholines, most of the polyphenols, amino acids, and polyamines had a higher content in Yunnan province, and low-unsaturation-degree galactolipids, triacylglycerols, glucosylceramides with trihydroxy long-chain bases, acylated sterol glucosides, and some organic acids were more abundant in Henan province. Correlation analysis between differential metabolites and climatic factors indicated the vital importance of temperature. The fatty acid unsaturation degree of galactolipids could be influenced by temperature. Accumulation of polyphenols and decreases in the ratios of stigmasterols to sitosterols and glucosylstigmasterols to glucosylsitosterols were also correlated with lower temperature in Yunnan province. Furthermore, lipids were more sensitive to climatic variations than other metabolites.
关键词[WOS]: TERT-BUTYL ETHER ;  COLD-ACCLIMATION ;  SIMULTANEOUS EXTRACTION ;  PLANT METABOLOMICS ;  GENE-EXPRESSION ;  DROUGHT STRESS ;  ARABIDOPSIS ;  MEMBRANE ;  REVEALS ;  IDENTIFICATION
语种: 英语
WOS记录号: WOS:000356461800012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/146319
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Li, Lili,Lu, Xin,Zhao, Jieyu,et al. Lipidome and metabolome analysis of fresh tobacco leaves in different geographical regions using liquid chromatography-mass spectrometry[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2015,407(17):5009-5020.
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