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题名: Effects of pre-analytical processes on blood samples used in metabolomics studies
作者: Yin, Peiyuan1;  Lehmann, Rainer2, 3, 4;  Xu, Guowang1
关键词: Metabolomics ;  Preanalytical ;  Bias ;  Blood ;  Urine ;  Serum ;  Plasma ;  Liquid chromatography-mass spectrometry ;  Standard operation procedure ;  Biobank
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2015-07-01
DOI: 10.1007/s00216-015-8565-x
卷: 407, 期:17, 页:4879-4892
收录类别: SCI
文章类型: Review
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Every day, analytical and bio-analytical chemists make sustained efforts to improve the sensitivity, specificity, robustness, and reproducibility of their methods. Especially in targeted and non-targeted profiling approaches, including metabolomics analysis, these objectives are not easy to achieve; however, robust and reproducible measurements and low coefficients of variation (CV) are crucial for successful metabolomics approaches. Nevertheless, all efforts from the analysts are in vain if the sample quality is poor, i.e. if preanalytical errors are made by the partner during sample collection. Preanalytical risks and errors are more common than expected, even when standard operating procedures (SOP) are used. This risk is particularly high in clinical studies, and poor sample quality may heavily bias the CV of the final analytical results, leading to disappointing outcomes of the study and consequently, although unjustified, to critical questions about the analytical performance of the approach from the partner who provided the samples. This review focuses on the preanalytical phase of liquid chromatography-mass spectrometry-driven metabolomics analysis of body fluids. Several important preanalytical factors that may seriously affect the profile of the investigated metabolome in body fluids, including factors before sample collection, blood drawing, subsequent handling of the whole blood (transportation), processing of plasma and serum, and inadequate conditions for sample storage, will be discussed. In addition, a detailed description of latent effects on the stability of the blood metabolome and a suggestion for a practical procedure to circumvent risks in the preanalytical phase will be given.
关键词[WOS]: CHROMATOGRAPHY-MASS SPECTROMETRY ;  FREEZE-THAW CYCLES ;  INTERNAL BODY TIME ;  -20 DEGREES-C ;  LIQUID-CHROMATOGRAPHY ;  HUMAN PLASMA ;  CLINICAL-APPLICATIONS ;  POTENTIAL BIOMARKERS ;  METABOLITE PROFILES ;  GAS-CHROMATOGRAPHY
语种: 英语
WOS记录号: WOS:000356461800004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/146320
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Tubingen Hosp, Div Clin Chem & Pathobiochem, Cent Lab, D-72076 Tubingen, Germany
3.Univ Tubingen, Inst Diabet Res & Metab Dis IDM, Helmholtz Ctr Munich, D-72076 Tubingen, Germany
4.German Ctr Diabet Res DZD, D-72076 Tubingen, Germany

Recommended Citation:
Yin, Peiyuan,Lehmann, Rainer,Xu, Guowang. Effects of pre-analytical processes on blood samples used in metabolomics studies[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2015,407(17):4879-4892.
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