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Metabolomic profiling of emodin-induced cytotoxicity in human liver cells and mechanistic study
Liu, Xiaoyan1,2; Liu, Yanqiu3; Qu, Yang1; Cheng, Mengchun1; Xiao, Hongbin1,4
刊名TOXICOLOGY RESEARCH
2015
DOI10.1039/c4tx00246f
4期:4页:948-955
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Toxicology
研究领域[WOS]Toxicology
关键词[WOS]FATTY-ACID OXIDATION ; CHROMATOGRAPHY-MASS SPECTROMETRY ; ALOE-EMODIN ; INDUCED APOPTOSIS ; L-02 CELLS ; INDUCED HEPATOTOXICITY ; GENE-EXPRESSION ; PROTEIN ; DIFFERENTIATION ; ACETAMINOPHEN
英文摘要Emodin is one of the most representative natural anthraquinone polyphenols and the liver is one of the major target organs for drug-induced toxicology. The hepatocyte is frequently affected due to its role in emodin metabolism and accumulation. Although the hepatotoxicity of emodin has been reported, its toxicological mechanism is still unclear. The purpose of the present study was to evaluate the cytotoxicity of emodin in cultured human normal liver cells (L-02), to investigate the toxicity-related metabolic pathways and to predict the possible toxicity mechanism. Cell viability was analyzed by 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cytotoxicity tests demonstrated a concentration-dependent toxic effect of emodin on L-02 cells. Cells were treated for 48 h with low, medium and high doses of emodin, respectively, and then subjected to metabolomics analysis using ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS). Intracellular metabolomics analysis revealed that emodin significantly disturbed cellular glutathione and fatty acid metabolism. In addition, an emodin-cysteine adduct was identified in cell culture medium, and its level increased with increasing concentrations of emodin. The possible relationship among metabolic disorders, adduct formation and emodin hepatotoxicity was also discussed. This study provides new insight into the cytotoxicity of emodin on metabolic pathways in human liver cells.
语种英语
WOS记录号WOS:000356612300017
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/146324
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Dalian Med Univ, Coll Inst Integrat Med, Dalian 116044, Peoples R China
4.Beijing Univ Chinese Med, Beijing 100029, Peoples R China
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Liu, Xiaoyan,Liu, Yanqiu,Qu, Yang,et al. Metabolomic profiling of emodin-induced cytotoxicity in human liver cells and mechanistic study[J]. TOXICOLOGY RESEARCH,2015,4(4):948-955.
APA Liu, Xiaoyan,Liu, Yanqiu,Qu, Yang,Cheng, Mengchun,&Xiao, Hongbin.(2015).Metabolomic profiling of emodin-induced cytotoxicity in human liver cells and mechanistic study.TOXICOLOGY RESEARCH,4(4),948-955.
MLA Liu, Xiaoyan,et al."Metabolomic profiling of emodin-induced cytotoxicity in human liver cells and mechanistic study".TOXICOLOGY RESEARCH 4.4(2015):948-955.
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