中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Metabolomic profiling of emodin-induced cytotoxicity in human liver cells and mechanistic study
作者: Liu, Xiaoyan1, 2;  Liu, Yanqiu3;  Qu, Yang1;  Cheng, Mengchun1;  Xiao, Hongbin1, 4
刊名: TOXICOLOGY RESEARCH
发表日期: 2015
DOI: 10.1039/c4tx00246f
卷: 4, 期:4, 页:948-955
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Toxicology
研究领域[WOS]: Toxicology
英文摘要: Emodin is one of the most representative natural anthraquinone polyphenols and the liver is one of the major target organs for drug-induced toxicology. The hepatocyte is frequently affected due to its role in emodin metabolism and accumulation. Although the hepatotoxicity of emodin has been reported, its toxicological mechanism is still unclear. The purpose of the present study was to evaluate the cytotoxicity of emodin in cultured human normal liver cells (L-02), to investigate the toxicity-related metabolic pathways and to predict the possible toxicity mechanism. Cell viability was analyzed by 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cytotoxicity tests demonstrated a concentration-dependent toxic effect of emodin on L-02 cells. Cells were treated for 48 h with low, medium and high doses of emodin, respectively, and then subjected to metabolomics analysis using ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS). Intracellular metabolomics analysis revealed that emodin significantly disturbed cellular glutathione and fatty acid metabolism. In addition, an emodin-cysteine adduct was identified in cell culture medium, and its level increased with increasing concentrations of emodin. The possible relationship among metabolic disorders, adduct formation and emodin hepatotoxicity was also discussed. This study provides new insight into the cytotoxicity of emodin on metabolic pathways in human liver cells.
关键词[WOS]: FATTY-ACID OXIDATION ;  CHROMATOGRAPHY-MASS SPECTROMETRY ;  ALOE-EMODIN ;  INDUCED APOPTOSIS ;  L-02 CELLS ;  INDUCED HEPATOTOXICITY ;  GENE-EXPRESSION ;  PROTEIN ;  DIFFERENTIATION ;  ACETAMINOPHEN
语种: 英语
WOS记录号: WOS:000356612300017
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/146324
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Dalian Med Univ, Coll Inst Integrat Med, Dalian 116044, Peoples R China
4.Beijing Univ Chinese Med, Beijing 100029, Peoples R China

Recommended Citation:
Liu, Xiaoyan,Liu, Yanqiu,Qu, Yang,et al. Metabolomic profiling of emodin-induced cytotoxicity in human liver cells and mechanistic study[J]. TOXICOLOGY RESEARCH,2015,4(4):948-955.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Liu, Xiaoyan]'s Articles
 [Liu, Yanqiu]'s Articles
 [Qu, Yang]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Liu, Xiaoyan]‘s Articles
 [Liu, Yanqiu]‘s Articles
 [Qu, Yang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace