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Evaluation of separation properties of a modified strong cation exchange material named MEX and its application in 2D-MEX x C18 system to separate peptides from scorpion venom
Chen, Bo1; Xu, Junyan2; Fu, Qing1; Dong, Xuefang2; Guo, Zhimou2; Jin, Yu1; Liang, Xinmiao1,2
刊名ANALYST
2015
DOI10.1039/c5an00271k
140期:13页:4676-4686
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]TANDEM MASS-SPECTROMETRY ; BUTHUS-MARTENSI KARSCH ; 2-DIMENSIONAL LIQUID-CHROMATOGRAPHY ; STATIONARY-PHASE ; BASIC COMPOUNDS ; CHAIN PEPTIDES ; TOXINS ; IDENTIFICATION ; COLUMN ; HYDROPHOBICITY
英文摘要Peptides from scorpion venom represent one of the most promising drug sources for drug discovery for some specific diseases. Current challenges in their separation include high complexity, high homologies and the huge range of peptides. In this paper, a modified strong cation exchange material, named MEX, was utilised for the two- dimensional separation of peptides from complex scorpion venom. The silicabased MEX column was bonded with two functional groups; benzenesulfonic acid and cyanopropyl. To better understand its separation mechanisms, seven standard peptides with different properties were employed in an evaluation study, the results of which showed that two interactions were involved in the MEX column: electrostatic interactions based on benzenesulfonic acid groups dominated the separation of peptides; weak hydrophobic interactions introduced by cyanopropyl groups increased the column's selectivity for peptides with the same charge. This characteristic allowed the MEX column to overcome some of the drawbacks of traditional strong cation exchange (SCX) columns. Furthermore, the study showed the great effects of the acetonitrile (ACN) content, the sodium perchlorate (NaClO4) concentration and the buffer pH in the mobile phase on the peptides' retention and separation selectivity on the MEX column. Subsequently, the MEX column was combined with a C18 column to establish an off- line 2D- MEX x C18 system to separate peptides from scorpion Buthus martensi Karsch (BmK) venom. Due to complementary separation mechanisms in each dimension, a high orthogonality of 47.62% was achieved. Moreover, a good loading capacity, excellent stability and repeatability were exhibited by the MEX column, which are beneficial for its use in future preparation experiments. Therefore, the MEX column could be an alternative to the traditional SCX columns for the separation of peptides from scorpion venom.
语种英语
WOS记录号WOS:000356171400043
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/146328
专题中国科学院大连化学物理研究所
作者单位1.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
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GB/T 7714
Chen, Bo,Xu, Junyan,Fu, Qing,et al. Evaluation of separation properties of a modified strong cation exchange material named MEX and its application in 2D-MEX x C18 system to separate peptides from scorpion venom[J]. ANALYST,2015,140(13):4676-4686.
APA Chen, Bo.,Xu, Junyan.,Fu, Qing.,Dong, Xuefang.,Guo, Zhimou.,...&Liang, Xinmiao.(2015).Evaluation of separation properties of a modified strong cation exchange material named MEX and its application in 2D-MEX x C18 system to separate peptides from scorpion venom.ANALYST,140(13),4676-4686.
MLA Chen, Bo,et al."Evaluation of separation properties of a modified strong cation exchange material named MEX and its application in 2D-MEX x C18 system to separate peptides from scorpion venom".ANALYST 140.13(2015):4676-4686.
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