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题名: Ultrafast Tracking of a Single Live Virion During the Invagination of a Cell Membrane
作者: Pan, Yangang1, 2;  Wang, Shaowen2, 3;  Shan, Yuping1, 4, 6;  Zhang, Dinglin5;  Gao, Jing1, 2;  Zhang, Min1, 2;  Liu, Shuheng1;  Cai, Mingjun1;  Xu, Haijiao1;  Li, Guohui5;  Qin, Qiwei3;  Wang, Hongda1, 2
刊名: SMALL
发表日期: 2015-06-17
DOI: 10.1002/smll.201403491
卷: 11, 期:23, 页:2782-2788
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences ;  Technology
类目[WOS]: Chemistry, Multidisciplinary ;  Chemistry, Physical ;  Nanoscience & Nanotechnology ;  Materials Science, Multidisciplinary ;  Physics, Applied ;  Physics, Condensed Matter
研究领域[WOS]: Chemistry ;  Science & Technology - Other Topics ;  Materials Science ;  Physics
英文摘要: The first step in most viral infections is the penetration of the cell membrane via endocytosis. However, the underlying mechanism of this important process has not been quantitatively characterized; for example, the velocity and force of a single virion during invagination remain unknown. Here, the endocytosis of a single live virion (Singapore grouper iridovirus, SGIV) through the apical membranes of a host cell is monitored by developing and using a novel ultrafast (at the microsecond level) tracking technique: force tracing. For the first time, these results unambiguously reveal that the maximum velocity during the cell entry of a single SGIV by membrane invagination is approximately 200 nm s(-1), the endocytic force is approximately 60.8 +/- 18.5 pN, and the binding energy density increases with the engulfment depth. This report utilizing high temporospatial resolution (subnanometer and microsecond levels) approaches provides new insight into the dynamic process of viral infection via endocytosis and the mechanism of membrane invagination at the single-particle level.
关键词[WOS]: SINGAPORE GROUPER IRIDOVIRUS ;  INDIVIDUAL INFLUENZA-VIRUSES ;  ATOMIC-FORCE MICROSCOPY ;  HOST-CELLS ;  PROTEOMIC ANALYSIS ;  SPOTTED GROUPER ;  ENTRY ;  ENDOCYTOSIS ;  MACROPINOCYTOSIS ;  SPECTROSCOPY
语种: 英语
WOS记录号: WOS:000356448300011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/146335
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
4.Univ S Carolina, Dept Chem & Biochem, Columbia, SC 29208 USA
5.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Dalian 116023, Liaoning, Peoples R China
6.Sch Chem & Life Sci, Adv Inst Mat Sci, Changchun 130012, Peoples R China

Recommended Citation:
Pan, Yangang,Wang, Shaowen,Shan, Yuping,et al. Ultrafast Tracking of a Single Live Virion During the Invagination of a Cell Membrane[J]. SMALL,2015,11(23):2782-2788.
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