DICP OpenIR
Ultrafast Tracking of a Single Live Virion During the Invagination of a Cell Membrane
Pan, Yangang1,2; Wang, Shaowen2,3; Shan, Yuping1,4,6; Zhang, Dinglin5; Gao, Jing1,2; Zhang, Min1,2; Liu, Shuheng1; Cai, Mingjun1; Xu, Haijiao1; Li, Guohui5; Qin, Qiwei3; Wang, Hongda1,2
Source PublicationSMALL
2015-06-17
DOI10.1002/smll.201403491
Volume11Issue:23Pages:2782-2788
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
WOS SubjectChemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter
WOS Research AreaChemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS KeywordSINGAPORE GROUPER IRIDOVIRUS ; INDIVIDUAL INFLUENZA-VIRUSES ; ATOMIC-FORCE MICROSCOPY ; HOST-CELLS ; PROTEOMIC ANALYSIS ; SPOTTED GROUPER ; ENTRY ; ENDOCYTOSIS ; MACROPINOCYTOSIS ; SPECTROSCOPY
AbstractThe first step in most viral infections is the penetration of the cell membrane via endocytosis. However, the underlying mechanism of this important process has not been quantitatively characterized; for example, the velocity and force of a single virion during invagination remain unknown. Here, the endocytosis of a single live virion (Singapore grouper iridovirus, SGIV) through the apical membranes of a host cell is monitored by developing and using a novel ultrafast (at the microsecond level) tracking technique: force tracing. For the first time, these results unambiguously reveal that the maximum velocity during the cell entry of a single SGIV by membrane invagination is approximately 200 nm s(-1), the endocytic force is approximately 60.8 +/- 18.5 pN, and the binding energy density increases with the engulfment depth. This report utilizing high temporospatial resolution (subnanometer and microsecond levels) approaches provides new insight into the dynamic process of viral infection via endocytosis and the mechanism of membrane invagination at the single-particle level.
Language英语
WOS IDWOS:000356448300011
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/146335
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
4.Univ S Carolina, Dept Chem & Biochem, Columbia, SC 29208 USA
5.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Dalian 116023, Liaoning, Peoples R China
6.Sch Chem & Life Sci, Adv Inst Mat Sci, Changchun 130012, Peoples R China
Recommended Citation
GB/T 7714
Pan, Yangang,Wang, Shaowen,Shan, Yuping,et al. Ultrafast Tracking of a Single Live Virion During the Invagination of a Cell Membrane[J]. SMALL,2015,11(23):2782-2788.
APA Pan, Yangang.,Wang, Shaowen.,Shan, Yuping.,Zhang, Dinglin.,Gao, Jing.,...&Wang, Hongda.(2015).Ultrafast Tracking of a Single Live Virion During the Invagination of a Cell Membrane.SMALL,11(23),2782-2788.
MLA Pan, Yangang,et al."Ultrafast Tracking of a Single Live Virion During the Invagination of a Cell Membrane".SMALL 11.23(2015):2782-2788.
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