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题名: Characterisation of oligosaccharides from Baizhu by HILIC-MS
作者: Lin, Zhe1;  Liu, Yan-Fang2;  Qu, Yang1;  Shi, Li-Ying2;  Dou, De-Qiang1;  Kuang, Hai-Xue3
关键词: inulin-type oligosaccharides ;  Atractylodes macrocephala Koidz ;  XAmide column ;  spleen lymphocyte transformation
刊名: NATURAL PRODUCT RESEARCH
发表日期: 2015-07-03
DOI: 10.1080/14786419.2014.995652
卷: 29, 期:13, 页:1194-1200
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences ;  Life Sciences & Biomedicine
类目[WOS]: Chemistry, Applied ;  Chemistry, Medicinal
研究领域[WOS]: Chemistry ;  Pharmacology & Pharmacy
英文摘要: Polysaccharides from Baizhu were separated by preparative hydrophilic interaction liquid chromatography on an XAmide column and its components were characterised as inulin-type polysaccharides with structures of alpha-D-glucopyranosyl-[-(1 -> 2) -beta-D-fructofuranosyl-](n-1)-(1 -> 2) -beta-D-fructofuranoside (n=3-20) by a combinatory application of electrospray-ionisation mass spectrometry, nuclear magnetic resonance and IR, as well as the chemical analysis of monosaccharide composition. In addition, the contents of nystose and 1F-fructofranosylnystose in the crude and purified Baizhu polysaccharides were determined to be 5.81%, 4.92% and 0.70%, 0.84% (w/w), respectively. In addition, MTT assay indicated that the Baizhu polysaccharides could effectively promote spleen lymphocyte transformation for the enhancement of organism immunity. It is for the first time that inulin-type polysaccharides were discovered in Baizhu and its immuno-enhancing activity was reported, which is a vigorous evidence to explain the efficacy of Baizhu.
关键词[WOS]: FRACTIONS
语种: 英语
WOS记录号: WOS:000356374300002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/146338
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Liaoning Univ Tradit Chinese Med, Coll Pharm, Dalian 116600, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Heilongjiang Univ Chinese Med, Coll Pharm, Harbin 150000, Peoples R China

Recommended Citation:
Lin, Zhe,Liu, Yan-Fang,Qu, Yang,et al. Characterisation of oligosaccharides from Baizhu by HILIC-MS[J]. NATURAL PRODUCT RESEARCH,2015,29(13):1194-1200.
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