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Characterisation of oligosaccharides from Baizhu by HILIC-MS
Lin, Zhe1; Liu, Yan-Fang2; Qu, Yang1; Shi, Li-Ying2; Dou, De-Qiang1; Kuang, Hai-Xue3
关键词Inulin-type Oligosaccharides Atractylodes Macrocephala Koidz Xamide Column Spleen Lymphocyte Transformation
刊名NATURAL PRODUCT RESEARCH
2015-07-03
DOI10.1080/14786419.2014.995652
29期:13页:1194-1200
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine
类目[WOS]Chemistry, Applied ; Chemistry, Medicinal
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]FRACTIONS
英文摘要Polysaccharides from Baizhu were separated by preparative hydrophilic interaction liquid chromatography on an XAmide column and its components were characterised as inulin-type polysaccharides with structures of alpha-D-glucopyranosyl-[-(1 -> 2) -beta-D-fructofuranosyl-](n-1)-(1 -> 2) -beta-D-fructofuranoside (n=3-20) by a combinatory application of electrospray-ionisation mass spectrometry, nuclear magnetic resonance and IR, as well as the chemical analysis of monosaccharide composition. In addition, the contents of nystose and 1F-fructofranosylnystose in the crude and purified Baizhu polysaccharides were determined to be 5.81%, 4.92% and 0.70%, 0.84% (w/w), respectively. In addition, MTT assay indicated that the Baizhu polysaccharides could effectively promote spleen lymphocyte transformation for the enhancement of organism immunity. It is for the first time that inulin-type polysaccharides were discovered in Baizhu and its immuno-enhancing activity was reported, which is a vigorous evidence to explain the efficacy of Baizhu.
语种英语
WOS记录号WOS:000356374300002
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/146338
专题中国科学院大连化学物理研究所
作者单位1.Liaoning Univ Tradit Chinese Med, Coll Pharm, Dalian 116600, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Heilongjiang Univ Chinese Med, Coll Pharm, Harbin 150000, Peoples R China
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GB/T 7714
Lin, Zhe,Liu, Yan-Fang,Qu, Yang,et al. Characterisation of oligosaccharides from Baizhu by HILIC-MS[J]. NATURAL PRODUCT RESEARCH,2015,29(13):1194-1200.
APA Lin, Zhe,Liu, Yan-Fang,Qu, Yang,Shi, Li-Ying,Dou, De-Qiang,&Kuang, Hai-Xue.(2015).Characterisation of oligosaccharides from Baizhu by HILIC-MS.NATURAL PRODUCT RESEARCH,29(13),1194-1200.
MLA Lin, Zhe,et al."Characterisation of oligosaccharides from Baizhu by HILIC-MS".NATURAL PRODUCT RESEARCH 29.13(2015):1194-1200.
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