DICP OpenIR
Metabolomics Approach Reveals Integrated Metabolic Network Associated with Serotonin Deficiency
Weng, Rui1; Shen, Sensen1; Tian, Yonglu2; Burton, Casey3,4; Xu, Xinyuan1; Liu, Yi1; Chang, Cuilan1; Bai, Yu1; Liu, Huwei1
Source PublicationSCIENTIFIC REPORTS
2015-07-08
DOI10.1038/srep11864
Volume5
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology
WOS SubjectMultidisciplinary Sciences
WOS Research AreaScience & Technology - Other Topics
WOS KeywordPARKINSONS-DISEASE ; ALZHEIMERS-DISEASE ; BRAIN-SEROTONIN ; TRYPTOPHAN-METABOLISM ; PSYCHIATRIC-DISORDERS ; P-CHLOROPHENYLALANINE ; TARGETED METABOLOMICS ; DRUG DEVELOPMENT ; ACID LEVELS ; URIC-ACID
AbstractSerotonin is an important neurotransmitter that broadly participates in various biological processes. While serotonin deficiency has been associated with multiple pathological conditions such as depression, schizophrenia, Alzheimer's disease and Parkinson's disease, the serotonin-dependent mechanisms remain poorly understood. This study therefore aimed to identify novel biomarkers and metabolic pathways perturbed by serotonin deficiency using metabolomics approach in order to gain new metabolic insights into the serotonin deficiency-related molecular mechanisms. Serotonin deficiency was achieved through pharmacological inhibition of tryptophan hydroxylase (Tph) using p-chlorophenylalanine (pCPA) or genetic knockout of the neuronal specific Tph2 isoform. This dual approach improved specificity for the serotonin deficiency-associated biomarkers while minimizing nonspecific effects of pCPA treatment or Tph2 knockout (Tph2-/-). Non-targeted metabolic profiling and a targeted pCPA dose-response study identified 21 biomarkers in the pCPA-treated mice while 17 metabolites in the Tph2-/- mice were found to be significantly altered compared with the control mice. These newly identified biomarkers were associated with amino acid, energy, purine, lipid and gut microflora metabolisms. Oxidative stress was also found to be significantly increased in the serotonin deficient mice. These new biomarkers and the overall metabolic pathways may provide new understanding for the serotonin deficiency-associated mechanisms under multiple pathological states.
Language英语
WOS IDWOS:000357576800001
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/146383
Collection中国科学院大连化学物理研究所
Affiliation1.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Inst Analyt Chem,Coll Chem & Mol Engn, Beijing 100871, Peoples R China
2.Peking Univ, Lab Anim Ctr, Beijing 100871, Peoples R China
3.Missouri Univ Sci & Technol, Dept Chem, Rolla, MO 65409 USA
4.Missouri Univ Sci & Technol, Ctr Biomed Sci & Engn, Rolla, MO 65409 USA
Recommended Citation
GB/T 7714
Weng, Rui,Shen, Sensen,Tian, Yonglu,et al. Metabolomics Approach Reveals Integrated Metabolic Network Associated with Serotonin Deficiency[J]. SCIENTIFIC REPORTS,2015,5.
APA Weng, Rui.,Shen, Sensen.,Tian, Yonglu.,Burton, Casey.,Xu, Xinyuan.,...&Liu, Huwei.(2015).Metabolomics Approach Reveals Integrated Metabolic Network Associated with Serotonin Deficiency.SCIENTIFIC REPORTS,5.
MLA Weng, Rui,et al."Metabolomics Approach Reveals Integrated Metabolic Network Associated with Serotonin Deficiency".SCIENTIFIC REPORTS 5(2015).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Weng, Rui]'s Articles
[Shen, Sensen]'s Articles
[Tian, Yonglu]'s Articles
Baidu academic
Similar articles in Baidu academic
[Weng, Rui]'s Articles
[Shen, Sensen]'s Articles
[Tian, Yonglu]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Weng, Rui]'s Articles
[Shen, Sensen]'s Articles
[Tian, Yonglu]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.