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题名: Preparative separation of a challenging anthocyanin from Lycium ruthenicum Murr. by two-dimensional reversed-phase liquid chromatography/hydrophilic interaction chromatography
作者: Jin, Hongli1, 2;  Zhao, Jianqiang3;  Zhou, Weijia1;  Shen, Aijin1;  Yang, Fan1;  Liu, Yanfang1;  Guo, Zhimou1;  Zhang, Xiuli1;  Tao, Yanduo3;  Peng, Xiaojun2;  Liang, Xinmiao1
刊名: RSC ADVANCES
发表日期: 2015
DOI: 10.1039/c5ra08713a
卷: 5, 期:76, 页:62134-62141
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: The preparative separation of anthocyanins by HPLC often suffers from insufficient separation selectivity. In this work, a two-dimensional liquid (LC-LC) method was established to efficiently purify a challenging anthocyanin in Lycium ruthenicum Murray. Reversed phase liquid chromatography (RPLC) was used in the first-dimension preparation to fractionate the sample for its high separation efficiency. After the optimization of second-dimension methods, hydrophilic interaction chromatography (HILIC) was applied to further isolate the anthocyanin for the good orthogonality to RPLC. To improve HILIC separation for anthocyanins, stationary phases and mobile phases were investigated systematically. A satisfactory result was obtained on a zwitterionic Click XIon column with 1% phosphoric acid as an acidic additive. Using the above method, the anthocyanin and three new alkaloids were isolated from L. ruthenicum for the first time. This RPLC/HILIC method solved the coelution problem of anthocyanin and basic non-anthocyanins in one-dimensional HPLC, benefiting from the significantly improved separation resolution.
关键词[WOS]: HYDROPHILIC-INTERACTION CHROMATOGRAPHY ;  CHARGED C18 COLUMN ;  STATIONARY-PHASE ;  MASS-SPECTROMETRY ;  POLAR COMPOUNDS ;  PURIFICATION ;  BUFADIENOLIDES ;  PEPTIDES ;  EXTRACTS ;  SKIN
语种: 英语
WOS记录号: WOS:000358353100077
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/146412
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China
3.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Peoples R China

Recommended Citation:
Jin, Hongli,Zhao, Jianqiang,Zhou, Weijia,et al. Preparative separation of a challenging anthocyanin from Lycium ruthenicum Murr. by two-dimensional reversed-phase liquid chromatography/hydrophilic interaction chromatography[J]. RSC ADVANCES,2015,5(76):62134-62141.
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