DICP OpenIR
Preparative separation of a challenging anthocyanin from Lycium ruthenicum Murr. by two-dimensional reversed-phase liquid chromatography/hydrophilic interaction chromatography
Jin, Hongli1,2; Zhao, Jianqiang3; Zhou, Weijia1; Shen, Aijin1; Yang, Fan1; Liu, Yanfang1; Guo, Zhimou1; Zhang, Xiuli1; Tao, Yanduo3; Peng, Xiaojun2; Liang, Xinmiao1
Source PublicationRSC ADVANCES
2015
DOI10.1039/c5ra08713a
Volume5Issue:76Pages:62134-62141
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordHYDROPHILIC-INTERACTION CHROMATOGRAPHY ; CHARGED C18 COLUMN ; STATIONARY-PHASE ; MASS-SPECTROMETRY ; POLAR COMPOUNDS ; PURIFICATION ; BUFADIENOLIDES ; PEPTIDES ; EXTRACTS ; SKIN
AbstractThe preparative separation of anthocyanins by HPLC often suffers from insufficient separation selectivity. In this work, a two-dimensional liquid (LC-LC) method was established to efficiently purify a challenging anthocyanin in Lycium ruthenicum Murray. Reversed phase liquid chromatography (RPLC) was used in the first-dimension preparation to fractionate the sample for its high separation efficiency. After the optimization of second-dimension methods, hydrophilic interaction chromatography (HILIC) was applied to further isolate the anthocyanin for the good orthogonality to RPLC. To improve HILIC separation for anthocyanins, stationary phases and mobile phases were investigated systematically. A satisfactory result was obtained on a zwitterionic Click XIon column with 1% phosphoric acid as an acidic additive. Using the above method, the anthocyanin and three new alkaloids were isolated from L. ruthenicum for the first time. This RPLC/HILIC method solved the coelution problem of anthocyanin and basic non-anthocyanins in one-dimensional HPLC, benefiting from the significantly improved separation resolution.
Language英语
WOS IDWOS:000358353100077
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/146412
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China
3.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Peoples R China
Recommended Citation
GB/T 7714
Jin, Hongli,Zhao, Jianqiang,Zhou, Weijia,et al. Preparative separation of a challenging anthocyanin from Lycium ruthenicum Murr. by two-dimensional reversed-phase liquid chromatography/hydrophilic interaction chromatography[J]. RSC ADVANCES,2015,5(76):62134-62141.
APA Jin, Hongli.,Zhao, Jianqiang.,Zhou, Weijia.,Shen, Aijin.,Yang, Fan.,...&Liang, Xinmiao.(2015).Preparative separation of a challenging anthocyanin from Lycium ruthenicum Murr. by two-dimensional reversed-phase liquid chromatography/hydrophilic interaction chromatography.RSC ADVANCES,5(76),62134-62141.
MLA Jin, Hongli,et al."Preparative separation of a challenging anthocyanin from Lycium ruthenicum Murr. by two-dimensional reversed-phase liquid chromatography/hydrophilic interaction chromatography".RSC ADVANCES 5.76(2015):62134-62141.
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