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Hydrophilic interaction liquid chromatography for the separation, purification, and quantification of raffinose family oligosaccharides from Lycopus lucidus Turcz
Liang, Tu1; Fu, Qing1; Li, Fangbing1; Zhou, Wei1; Xin, Huaxia1; Wang, Hui1; Jin, Yu1; Liang, Xinmiao1,2
关键词Hydrophilic Interaction Liquid Chromatography Lycopus Lucidus Turcz. Oligosaccharides Purification Quantitation
刊名JOURNAL OF SEPARATION SCIENCE
2015-08-01
DOI10.1002/jssc.201500316
38期:15页:2607-2613
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]MASS-SPECTROMETRY ; STATIONARY-PHASE ; SEEDS ; TIME ; HPLC ; L.
英文摘要A systematic strategy based on hydrophilic interaction liquid chromatography was developed for the separation, purification and quantification of raffinose family oligosaccharides from Lycopus lucidus Turcz. Methods with enough hydrophilicity and selectivity were utilized to resolve the problems encountered in the separation of oligosaccharides such as low retention, low resolution and poor solubility. The raffinose family oligosaccharides in L. lucidus Turcz. were isolated using solid-phase extraction followed by hydrophilic interaction liquid chromatography at semi-preparative scale to obtain standards of stachyose, verbascose and ajugose. Utilizing the obtained oligosaccharides as standards, a quantitative determination method was developed, validated and applied for the content determination of raffinose family oligosaccharides both in the aerial and root parts of L. lucidus Turcz. There were no oligosaccharides in the aerial parts, while in the root parts, the total content was 686.5 mg/g with the average distribution: raffinose 66.5 mg/g, stachyose 289.0 mg/g, verbascose 212.4 mg/g, and ajugose 118.6 mg/g. The result provided the potential of roots of L. lucidus Turcz. as new raffinose family oligosaccharides sources for functional food. Moreover, since the present systematic strategy is efficient, sensitive and robust, separation, purification and quantification of oligosaccharides by hydrophilic interaction liquid chromatography seems to be possible.
语种英语
WOS记录号WOS:000358443600006
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被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/146419
专题中国科学院大连化学物理研究所
作者单位1.E China Univ Sci & Technol, Sch Pharm, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Shanghai 200237, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Key Lab Nat Med, Dalian 116023, Peoples R China
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GB/T 7714
Liang, Tu,Fu, Qing,Li, Fangbing,et al. Hydrophilic interaction liquid chromatography for the separation, purification, and quantification of raffinose family oligosaccharides from Lycopus lucidus Turcz[J]. JOURNAL OF SEPARATION SCIENCE,2015,38(15):2607-2613.
APA Liang, Tu.,Fu, Qing.,Li, Fangbing.,Zhou, Wei.,Xin, Huaxia.,...&Liang, Xinmiao.(2015).Hydrophilic interaction liquid chromatography for the separation, purification, and quantification of raffinose family oligosaccharides from Lycopus lucidus Turcz.JOURNAL OF SEPARATION SCIENCE,38(15),2607-2613.
MLA Liang, Tu,et al."Hydrophilic interaction liquid chromatography for the separation, purification, and quantification of raffinose family oligosaccharides from Lycopus lucidus Turcz".JOURNAL OF SEPARATION SCIENCE 38.15(2015):2607-2613.
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