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Hydrophilic interaction liquid chromatography for the separation, purification, and quantification of raffinose family oligosaccharides from Lycopus lucidus Turcz
Liang, Tu1; Fu, Qing1; Li, Fangbing1; Zhou, Wei1; Xin, Huaxia1; Wang, Hui1; Jin, Yu1; Liang, Xinmiao1,2
KeywordHydrophilic Interaction Liquid Chromatography Lycopus Lucidus Turcz. Oligosaccharides Purification Quantitation
Source PublicationJOURNAL OF SEPARATION SCIENCE
2015-08-01
DOI10.1002/jssc.201500316
Volume38Issue:15Pages:2607-2613
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordMASS-SPECTROMETRY ; STATIONARY-PHASE ; SEEDS ; TIME ; HPLC ; L.
AbstractA systematic strategy based on hydrophilic interaction liquid chromatography was developed for the separation, purification and quantification of raffinose family oligosaccharides from Lycopus lucidus Turcz. Methods with enough hydrophilicity and selectivity were utilized to resolve the problems encountered in the separation of oligosaccharides such as low retention, low resolution and poor solubility. The raffinose family oligosaccharides in L. lucidus Turcz. were isolated using solid-phase extraction followed by hydrophilic interaction liquid chromatography at semi-preparative scale to obtain standards of stachyose, verbascose and ajugose. Utilizing the obtained oligosaccharides as standards, a quantitative determination method was developed, validated and applied for the content determination of raffinose family oligosaccharides both in the aerial and root parts of L. lucidus Turcz. There were no oligosaccharides in the aerial parts, while in the root parts, the total content was 686.5 mg/g with the average distribution: raffinose 66.5 mg/g, stachyose 289.0 mg/g, verbascose 212.4 mg/g, and ajugose 118.6 mg/g. The result provided the potential of roots of L. lucidus Turcz. as new raffinose family oligosaccharides sources for functional food. Moreover, since the present systematic strategy is efficient, sensitive and robust, separation, purification and quantification of oligosaccharides by hydrophilic interaction liquid chromatography seems to be possible.
Language英语
WOS IDWOS:000358443600006
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/146419
Collection中国科学院大连化学物理研究所
Affiliation1.E China Univ Sci & Technol, Sch Pharm, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Shanghai 200237, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Key Lab Nat Med, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Liang, Tu,Fu, Qing,Li, Fangbing,et al. Hydrophilic interaction liquid chromatography for the separation, purification, and quantification of raffinose family oligosaccharides from Lycopus lucidus Turcz[J]. JOURNAL OF SEPARATION SCIENCE,2015,38(15):2607-2613.
APA Liang, Tu.,Fu, Qing.,Li, Fangbing.,Zhou, Wei.,Xin, Huaxia.,...&Liang, Xinmiao.(2015).Hydrophilic interaction liquid chromatography for the separation, purification, and quantification of raffinose family oligosaccharides from Lycopus lucidus Turcz.JOURNAL OF SEPARATION SCIENCE,38(15),2607-2613.
MLA Liang, Tu,et al."Hydrophilic interaction liquid chromatography for the separation, purification, and quantification of raffinose family oligosaccharides from Lycopus lucidus Turcz".JOURNAL OF SEPARATION SCIENCE 38.15(2015):2607-2613.
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