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Expression and characterization of a lytic polysaccharide monooxygenase from Bacillus thuringiensis
Zhang, Huiyan1,2; Zhao, Yong2; Cao, Hailong2; Mou, Guangqing1; Yin, Heng2
关键词B. Thuringiensis Lytic Polysaccharide Monooxygenases Polysaccharides
刊名INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
2015-08-01
DOI10.1016/j.ijbiomac.2015.04.054
79页:72-75
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]CELLULOSE ; PROTEIN ; ENZYMES
英文摘要Lytic polysaccharide monooxygenases (LPMOs) are recently discovered oxidative enzymes that are capable of oxidative cleavage of recalcitrant polysaccharides such as chitin or cellulose. Despite the importance of LPMOs in biomass conversion and the large number of lpmo genes in microorganisms, only a few LPMOs have been well studied, and further characterization of these proteins is thus of interest. In this study, a chitin-active AA10 family LPMO from Bacillus thuringiensis subsp. kurstaki, BtLPMO10A, is described. This enzyme generates even-numbered oxidized oligosaccharides as the dominated products from crystalline chitin, however, interestingly, when colloidal chitin is used as the substrate, a ladder of oxidized oligosaccharides is observed. These results provide new insights into the action mode of LPMOs that may be affected by the substrates. (C) 2015 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000359166300010
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/146448
专题中国科学院大连化学物理研究所
作者单位1.Dalian Polytech Univ, Sch Food Sci & Technol, Dalian 116034, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
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Zhang, Huiyan,Zhao, Yong,Cao, Hailong,et al. Expression and characterization of a lytic polysaccharide monooxygenase from Bacillus thuringiensis[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2015,79:72-75.
APA Zhang, Huiyan,Zhao, Yong,Cao, Hailong,Mou, Guangqing,&Yin, Heng.(2015).Expression and characterization of a lytic polysaccharide monooxygenase from Bacillus thuringiensis.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,79,72-75.
MLA Zhang, Huiyan,et al."Expression and characterization of a lytic polysaccharide monooxygenase from Bacillus thuringiensis".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 79(2015):72-75.
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