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Expression and characterization of a lytic polysaccharide monooxygenase from Bacillus thuringiensis
Zhang, Huiyan1,2; Zhao, Yong2; Cao, Hailong2; Mou, Guangqing1; Yin, Heng2
KeywordB. Thuringiensis Lytic Polysaccharide Monooxygenases Polysaccharides
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
2015-08-01
DOI10.1016/j.ijbiomac.2015.04.054
Volume79Pages:72-75
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemistry & Molecular Biology
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordCELLULOSE ; PROTEIN ; ENZYMES
AbstractLytic polysaccharide monooxygenases (LPMOs) are recently discovered oxidative enzymes that are capable of oxidative cleavage of recalcitrant polysaccharides such as chitin or cellulose. Despite the importance of LPMOs in biomass conversion and the large number of lpmo genes in microorganisms, only a few LPMOs have been well studied, and further characterization of these proteins is thus of interest. In this study, a chitin-active AA10 family LPMO from Bacillus thuringiensis subsp. kurstaki, BtLPMO10A, is described. This enzyme generates even-numbered oxidized oligosaccharides as the dominated products from crystalline chitin, however, interestingly, when colloidal chitin is used as the substrate, a ladder of oxidized oligosaccharides is observed. These results provide new insights into the action mode of LPMOs that may be affected by the substrates. (C) 2015 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000359166300010
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/146448
Collection中国科学院大连化学物理研究所
Affiliation1.Dalian Polytech Univ, Sch Food Sci & Technol, Dalian 116034, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Huiyan,Zhao, Yong,Cao, Hailong,et al. Expression and characterization of a lytic polysaccharide monooxygenase from Bacillus thuringiensis[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2015,79:72-75.
APA Zhang, Huiyan,Zhao, Yong,Cao, Hailong,Mou, Guangqing,&Yin, Heng.(2015).Expression and characterization of a lytic polysaccharide monooxygenase from Bacillus thuringiensis.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,79,72-75.
MLA Zhang, Huiyan,et al."Expression and characterization of a lytic polysaccharide monooxygenase from Bacillus thuringiensis".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 79(2015):72-75.
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