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题名: Role of three-dimensional matrix stiffness in regulating the chemoresistance of hepatocellular carcinoma cells
作者: Liu, Chang1, 2;  Liu, Yang1;  Xie, Hong-guo1;  Zhao, Shan1;  Xu, Xiao-xi1, 2;  Fan, Li-xin3;  Guo, Xin1;  Lu, Ting4;  Sun, Guang-Wei1;  Ma, Xiao-jun1
关键词: alginate gel (ALG) beads ;  matrix stiffness ;  drug resistance ;  response surface methodology (RSM)
刊名: BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
发表日期: 2015-07-01
DOI: 10.1002/bab.1302
卷: 62, 期:4, 页:556-562
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology
英文摘要: Hepatocellular carcinoma (HCC) was the most common primary liver cancer, and its resistance to anti-tumor drugs often caused the death of patients suffering with HCC. Matrix stiffness was reported to be closely related to tumor chemoresistance; however, the relationship between HCC drug resistance and three-dimensional (3D) matrix stiffness is still unclear at present. In this study, alginate gel (ALG) beads with controllable matrix stiffness were used to mimic tumor tissue rigidity, and the role of 3D matrix stiffness in regulating the chemoresistance of HCC cells was investigated by using these ALG beads. It was found that HCC cells in ALG beads with 105 kPa stiffness had highest resistance to paclitaxel, 5-FU, and cisplatin. Although the mechanism was still uncovered, ABC transporters and endoplasmic reticulum stress-related molecules were highly expressed in ALG bead-encapsulated HCC cells compared with two-dimensional-cultured cells, which suggested a very complex mechanism underlying HCC drug resistance in 3D culture conditions. In addition, to mimic the specific stiffness of HCC tumor tissue, or other tumor tissues in vivo, response surface methodology (RSM) was used to build up a prediction mathematical model so that ALG beads with desired matrix stiffness could be prepared by simply changing three factors: molecular weight, G content, and alginate concentration.
关键词[WOS]: ENDOPLASMIC-RETICULUM STRESS ;  LUNG-CANCER CELLS ;  DRUG-RESISTANCE ;  IN-VITRO ;  TRANSIENT ELASTOGRAPHY ;  GROWTH ;  MECHANISMS ;  EXPRESSION ;  APOPTOSIS ;  SUBSTRATE
语种: 英语
WOS记录号: WOS:000360229200016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/146496
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Third Peoples Hosp Dalian, Dept Oncol, Dalian, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China

Recommended Citation:
Liu, Chang,Liu, Yang,Xie, Hong-guo,et al. Role of three-dimensional matrix stiffness in regulating the chemoresistance of hepatocellular carcinoma cells[J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,2015,62(4):556-562.
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