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Role of three-dimensional matrix stiffness in regulating the chemoresistance of hepatocellular carcinoma cells
Liu, Chang1,2; Liu, Yang1; Xie, Hong-guo1; Zhao, Shan1; Xu, Xiao-xi1,2; Fan, Li-xin3; Guo, Xin1; Lu, Ting4; Sun, Guang-Wei1; Ma, Xiao-jun1
KeywordAlginate Gel (Alg) Beads Matrix Stiffness Drug Resistance Response Surface Methodology (Rsm)
Source PublicationBIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
2015-07-01
DOI10.1002/bab.1302
Volume62Issue:4Pages:556-562
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS KeywordENDOPLASMIC-RETICULUM STRESS ; LUNG-CANCER CELLS ; DRUG-RESISTANCE ; IN-VITRO ; TRANSIENT ELASTOGRAPHY ; GROWTH ; MECHANISMS ; EXPRESSION ; APOPTOSIS ; SUBSTRATE
AbstractHepatocellular carcinoma (HCC) was the most common primary liver cancer, and its resistance to anti-tumor drugs often caused the death of patients suffering with HCC. Matrix stiffness was reported to be closely related to tumor chemoresistance; however, the relationship between HCC drug resistance and three-dimensional (3D) matrix stiffness is still unclear at present. In this study, alginate gel (ALG) beads with controllable matrix stiffness were used to mimic tumor tissue rigidity, and the role of 3D matrix stiffness in regulating the chemoresistance of HCC cells was investigated by using these ALG beads. It was found that HCC cells in ALG beads with 105 kPa stiffness had highest resistance to paclitaxel, 5-FU, and cisplatin. Although the mechanism was still uncovered, ABC transporters and endoplasmic reticulum stress-related molecules were highly expressed in ALG bead-encapsulated HCC cells compared with two-dimensional-cultured cells, which suggested a very complex mechanism underlying HCC drug resistance in 3D culture conditions. In addition, to mimic the specific stiffness of HCC tumor tissue, or other tumor tissues in vivo, response surface methodology (RSM) was used to build up a prediction mathematical model so that ALG beads with desired matrix stiffness could be prepared by simply changing three factors: molecular weight, G content, and alginate concentration.
Language英语
WOS IDWOS:000360229200016
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/146496
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Third Peoples Hosp Dalian, Dept Oncol, Dalian, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Liu, Chang,Liu, Yang,Xie, Hong-guo,et al. Role of three-dimensional matrix stiffness in regulating the chemoresistance of hepatocellular carcinoma cells[J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,2015,62(4):556-562.
APA Liu, Chang.,Liu, Yang.,Xie, Hong-guo.,Zhao, Shan.,Xu, Xiao-xi.,...&Ma, Xiao-jun.(2015).Role of three-dimensional matrix stiffness in regulating the chemoresistance of hepatocellular carcinoma cells.BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,62(4),556-562.
MLA Liu, Chang,et al."Role of three-dimensional matrix stiffness in regulating the chemoresistance of hepatocellular carcinoma cells".BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY 62.4(2015):556-562.
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