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题名: The preservation of islet with alginate encapsulation in the process of transportation
作者: Li, Na1, 2, 3;  Zhang, Ying2;  Xiu, Zhilong1;  Wang, Yu2, 3;  Chen, Li2, 3;  Wang, Shujun1, 2;  Li, Shen1, 2;  Guo, Xin2;  Ma, Xiaojun2
关键词: Box-Behnken design ;  cell encapsulation ;  islet survival rate ;  islet transportation ;  response surface methodology
刊名: BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
发表日期: 2015-07-01
DOI: 10.1002/bab.1295
卷: 62, 期:4, 页:530-536
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology
英文摘要: Restoration of insulin secretion by transplantation of isolated islets is a treatment for type diabetes mellitus. One of the major issues with clinical treatment of islet transplantation is how to maintain islet viability during transportation from the donor to the patient. We developed a method that uses alginate encapsulation to protect islets from mechanical damage during shipment. We tested several variables for their impact on islet viability during transportation and used the significant variable to build a response surface methodology (RSM) model by the Box-Behnken design method. This type of model is a mathematical and statistical technique that we used to optimize the conditions for islet viability during shipment. In this study, the factors that significantly affected islet survival rate were incubation time, serum concentration, and preservation time. Then, an empirical model was built to optimize conditions of the islets for shipping according to the responses of the effect factors with RSM. This model can be used to predict the islet survival rate and can serve as a guide for optimizing the transportation method of islets and increasing the success rate of the transplant procedure.
关键词[WOS]: RAT ISLETS ;  CRYOPRESERVATION ;  TRANSPLANTATION ;  SURVIVAL ;  CELLS
语种: 英语
WOS记录号: WOS:000360229200013
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/146498
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China

Recommended Citation:
Li, Na,Zhang, Ying,Xiu, Zhilong,et al. The preservation of islet with alginate encapsulation in the process of transportation[J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,2015,62(4):530-536.
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