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The preservation of islet with alginate encapsulation in the process of transportation
Li, Na1,2,3; Zhang, Ying2; Xiu, Zhilong1; Wang, Yu2,3; Chen, Li2,3; Wang, Shujun1,2; Li, Shen1,2; Guo, Xin2; Ma, Xiaojun2
关键词Box-behnken Design Cell Encapsulation Islet Survival Rate Islet Transportation Response Surface Methodology
刊名BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
2015-07-01
DOI10.1002/bab.1295
62期:4页:530-536
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]RAT ISLETS ; CRYOPRESERVATION ; TRANSPLANTATION ; SURVIVAL ; CELLS
英文摘要Restoration of insulin secretion by transplantation of isolated islets is a treatment for type diabetes mellitus. One of the major issues with clinical treatment of islet transplantation is how to maintain islet viability during transportation from the donor to the patient. We developed a method that uses alginate encapsulation to protect islets from mechanical damage during shipment. We tested several variables for their impact on islet viability during transportation and used the significant variable to build a response surface methodology (RSM) model by the Box-Behnken design method. This type of model is a mathematical and statistical technique that we used to optimize the conditions for islet viability during shipment. In this study, the factors that significantly affected islet survival rate were incubation time, serum concentration, and preservation time. Then, an empirical model was built to optimize conditions of the islets for shipping according to the responses of the effect factors with RSM. This model can be used to predict the islet survival rate and can serve as a guide for optimizing the transportation method of islets and increasing the success rate of the transplant procedure.
语种英语
WOS记录号WOS:000360229200013
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/146498
专题中国科学院大连化学物理研究所
作者单位1.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Li, Na,Zhang, Ying,Xiu, Zhilong,et al. The preservation of islet with alginate encapsulation in the process of transportation[J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,2015,62(4):530-536.
APA Li, Na.,Zhang, Ying.,Xiu, Zhilong.,Wang, Yu.,Chen, Li.,...&Ma, Xiaojun.(2015).The preservation of islet with alginate encapsulation in the process of transportation.BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,62(4),530-536.
MLA Li, Na,et al."The preservation of islet with alginate encapsulation in the process of transportation".BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY 62.4(2015):530-536.
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