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Functional and structural properties of a novel cellulosome-like multienzyme complex: efficient glycoside hydrolysis of water-insoluble 7-xylosyl-10-deacetylpaclitaxel
Dou, Tong-Yi1,2; Luan, Hong-Wei1; Ge, Guang-Bo1; Dong, Ming-Ming3; Zou, Han-Fa3; He, Yu-Qi1; Cui, Pan1,2; Wang, Jia-Yue1,2; Hao, Da-Cheng4; Yang, Shi-Lin5; Yang, Ling1,5
Source PublicationSCIENTIFIC REPORTS
2015-09-08
DOI10.1038/srep13768
Volume5
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology
WOS SubjectMultidisciplinary Sciences
WOS Research AreaScience & Technology - Other Topics
WOS KeywordCELLULOLYTIC ENZYME COMPLEX ; CLOSTRIDIUM-THERMOCELLUM ; XYLOSIDASE ; BIOCATALYSIS ; ORGANIZATION ; HYDROLASES ; DATABASE ; TAXANES ; BARLEY
AbstractCellulosome is a kind of multienzyme complex that displays high activity, selectivity, and stability. Here, we report a novel, non-cellulolytic, cellulosome-like multienzyme complex that produced by the Cellulosimicrobium cellulans wild-type strain F16 isolated from soil microflora. This multienzyme complex, with excellent catalytic efficiency of k(cat) 13.2 s(-1) to remove the C-7 xylosyl group from 7-xylosyl-10-deacetylpaclitaxel (10-DAXP), has an outstanding tolerance against organic solvents and an excellent general stability, with the long half-life of 214 hours. This cellulosome-like multienzyme complex has a novel structure distinct from the well-documented ones. The key catalytic subunit responsible for the beta-xylosidase activity against 10-DAXP is identified to be a novel protein, indicating a new glycoside hydrolase (GH) family. The pioneering work described here offers a novel nanoscale biocatalyst for the production of biofuels and chemicals from renewable plant-based natural resources.
Language英语
WOS IDWOS:000360798000003
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/146512
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
4.Dalian Jiaotong Univ, Sch Environm & Chem Engn, Inst Biotechnol, Dalian 116028, Peoples R China
5.Jiangxi Univ Tradit Chinese Med, Nanchang 330004, Peoples R China
Recommended Citation
GB/T 7714
Dou, Tong-Yi,Luan, Hong-Wei,Ge, Guang-Bo,et al. Functional and structural properties of a novel cellulosome-like multienzyme complex: efficient glycoside hydrolysis of water-insoluble 7-xylosyl-10-deacetylpaclitaxel[J]. SCIENTIFIC REPORTS,2015,5.
APA Dou, Tong-Yi.,Luan, Hong-Wei.,Ge, Guang-Bo.,Dong, Ming-Ming.,Zou, Han-Fa.,...&Yang, Ling.(2015).Functional and structural properties of a novel cellulosome-like multienzyme complex: efficient glycoside hydrolysis of water-insoluble 7-xylosyl-10-deacetylpaclitaxel.SCIENTIFIC REPORTS,5.
MLA Dou, Tong-Yi,et al."Functional and structural properties of a novel cellulosome-like multienzyme complex: efficient glycoside hydrolysis of water-insoluble 7-xylosyl-10-deacetylpaclitaxel".SCIENTIFIC REPORTS 5(2015).
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