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题名: Functional and structural properties of a novel cellulosome-like multienzyme complex: efficient glycoside hydrolysis of water-insoluble 7-xylosyl-10-deacetylpaclitaxel
作者: Dou, Tong-Yi1, 2;  Luan, Hong-Wei1;  Ge, Guang-Bo1;  Dong, Ming-Ming3;  Zou, Han-Fa3;  He, Yu-Qi1;  Cui, Pan1, 2;  Wang, Jia-Yue1, 2;  Hao, Da-Cheng4;  Yang, Shi-Lin5;  Yang, Ling1, 5
刊名: SCIENTIFIC REPORTS
发表日期: 2015-09-08
DOI: 10.1038/srep13768
卷: 5
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
英文摘要: Cellulosome is a kind of multienzyme complex that displays high activity, selectivity, and stability. Here, we report a novel, non-cellulolytic, cellulosome-like multienzyme complex that produced by the Cellulosimicrobium cellulans wild-type strain F16 isolated from soil microflora. This multienzyme complex, with excellent catalytic efficiency of k(cat) 13.2 s(-1) to remove the C-7 xylosyl group from 7-xylosyl-10-deacetylpaclitaxel (10-DAXP), has an outstanding tolerance against organic solvents and an excellent general stability, with the long half-life of 214 hours. This cellulosome-like multienzyme complex has a novel structure distinct from the well-documented ones. The key catalytic subunit responsible for the beta-xylosidase activity against 10-DAXP is identified to be a novel protein, indicating a new glycoside hydrolase (GH) family. The pioneering work described here offers a novel nanoscale biocatalyst for the production of biofuels and chemicals from renewable plant-based natural resources.
关键词[WOS]: CELLULOLYTIC ENZYME COMPLEX ;  CLOSTRIDIUM-THERMOCELLUM ;  XYLOSIDASE ;  BIOCATALYSIS ;  ORGANIZATION ;  HYDROLASES ;  DATABASE ;  TAXANES ;  BARLEY
语种: 英语
WOS记录号: WOS:000360798000003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/146512
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
4.Dalian Jiaotong Univ, Sch Environm & Chem Engn, Inst Biotechnol, Dalian 116028, Peoples R China
5.Jiangxi Univ Tradit Chinese Med, Nanchang 330004, Peoples R China

Recommended Citation:
Dou, Tong-Yi,Luan, Hong-Wei,Ge, Guang-Bo,et al. Functional and structural properties of a novel cellulosome-like multienzyme complex: efficient glycoside hydrolysis of water-insoluble 7-xylosyl-10-deacetylpaclitaxel[J]. SCIENTIFIC REPORTS,2015,5.
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