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题名: Affinity purification in target identification: the specificity challenge
作者: Zheng, Wenlu1;  Li, Gang2;  Li, Xiaoyu1, 2
关键词: Target identification ;  Affinity purification ;  Small molecule ;  Phenotypic screening ;  Protein-ligand interaction ;  Specificity
刊名: ARCHIVES OF PHARMACAL RESEARCH
发表日期: 2015-09-01
DOI: 10.1007/s12272-015-0635-2
卷: 38, 期:9, 页:1661-1685
收录类别: SCI
文章类型: Review
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Chemistry, Medicinal ;  Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
英文摘要: Since phenotype-based screening directly evaluates capability of small molecules for modulating biology in actual biological systems, it has become an important discover modality in modern pharmaceutical sciences. However, in order to fully elucidate the molecular mechanism underlying the bioactivity of small molecules, identification of their biological targets is an indispensable step. Among the many target identification strategies developed during the past several decades, affinity purification remains to be one of the most important and powerful approaches, as it can directly reveal the physical interactions between small molecules and their biomolecular targets. However, due to the complexity of the proteome and the diversity of small molecule-protein interactions, affinity purification faces the specificity challenge: how to identify the true specific targets from the non-specific background? Focusing on this challenge, in this review, we briefly introduce the history and background of affinity purification, and then we discussed the major technological developments aiming to address this challenge. We have summarized these approaches in two categories: noise reduction and comparative distinction. This review also highlights the importance of choosing an integrated approach combining multiple methods to achieving success in target identification.
关键词[WOS]: PROTEIN-LIGAND INTERACTIONS ;  SMALL-MOLECULE PROBES ;  PHOTOAFFINITY CROSS-LINKING ;  BIOACTIVE SMALL MOLECULES ;  QUANTITATIVE CHEMICAL PROTEOMICS ;  HISTONE DEACETYLASE COMPLEXES ;  CARBOHYDRATE-BINDING PROTEINS ;  DIRECTED TOSYL CHEMISTRY ;  TAGGED TRIAZINE LIBRARY ;  A1 ADENOSINE RECEPTOR
语种: 英语
WOS记录号: WOS:000361445000011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/146546
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Peking Univ, Shenzhen Grad Sch, Sch Chem Biol & Biotechnol, Key Lab Chem Genom, Shenzhen 518055, Peoples R China
2.Peking Univ, Key Lab Bioorgan Chem & Mol Engn, Coll Chem & Mol Engn, Minist Educ,BNLMS, Beijing 100871, Peoples R China

Recommended Citation:
Zheng, Wenlu,Li, Gang,Li, Xiaoyu. Affinity purification in target identification: the specificity challenge[J]. ARCHIVES OF PHARMACAL RESEARCH,2015,38(9):1661-1685.
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