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题名: Amine Chemistry Method for Selective Enrichment of N-Linked Glycopeptides for Glycoproteomics Analysis
作者: Zhang, Zhang1, 2;  Sun, Deguang3;  Cong, Yuting1, 4;  Mao, Jiawei1, 2;  Huang, Junfeng1, 2;  Qin, Hongqiang1, 2;  Liu, Jing1, 2;  Huang, Guang1;  Wang, Liming3;  Ye, Mingliang1;  Zou, Hanfa1
关键词: glycoproteomics ;  glycopeptide enrichment ;  reductive amination reaction
刊名: JOURNAL OF PROTEOME RESEARCH
发表日期: 2015-09-01
DOI: 10.1021/acs.jproteome.5b00306
卷: 14, 期:9, 页:3892-3899
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: An amine chemistry method was developed for the extraction of N-glycopeptides using amine-functionalized beads for glycoproteomics analysis. Two reductive amination reactions between primary amine and aldehyde were employed in this approach. The first one was to block the primary amines in the peptides by addition of formaldehyde and sodium c-yanoborohydride into the peptide sample, and the second one was to couple the glycopeptides onto solid phase beads by incubating the glycopeptides containing aldehyde groups (oxidized by periodate) with the amine-functionalized beads in the presence of sodium cyanoborohydride. It was demonstrated that the blocking of primary amines in the peptides by the first reductive amination reaction prior to the periodate oxidation made the amine chemistry method very efficient and sensitive. This new method was validated by analysis of glycoprotein standards as well as proteome samples. It was found that this new method led to significant increase in the identification of N-glycosites compared with the conventional hydrazide chemistry method.
关键词[WOS]: SOLID-PHASE EXTRACTION ;  MASS-SPECTROMETRY ;  QUANTITATIVE PROTEOMICS ;  MAGNETIC NANOPARTICLES ;  HYDRAZIDE CHEMISTRY ;  REDUCTIVE AMINATION ;  MESOPOROUS SILICA ;  GLYCOSYLATION ;  QUANTIFICATION ;  OXIDATION
语种: 英语
WOS记录号: WOS:000361087100042
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/146549
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Dalian Med Univ, Affiliated Hosp 2, Dalian 116027, Peoples R China
4.Jilin Univ, Res Ctr Drug Metab, Sch Life Sci, Changchun 130012, Peoples R China

Recommended Citation:
Zhang, Zhang,Sun, Deguang,Cong, Yuting,et al. Amine Chemistry Method for Selective Enrichment of N-Linked Glycopeptides for Glycoproteomics Analysis[J]. JOURNAL OF PROTEOME RESEARCH,2015,14(9):3892-3899.
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