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Amine Chemistry Method for Selective Enrichment of N-Linked Glycopeptides for Glycoproteomics Analysis
Zhang, Zhang1,2; Sun, Deguang3; Cong, Yuting1,4; Mao, Jiawei1,2; Huang, Junfeng1,2; Qin, Hongqiang1,2; Liu, Jing1,2; Huang, Guang1; Wang, Liming3; Ye, Mingliang1; Zou, Hanfa1
KeywordGlycoproteomics Glycopeptide Enrichment Reductive Amination Reaction
Source PublicationJOURNAL OF PROTEOME RESEARCH
2015-09-01
DOI10.1021/acs.jproteome.5b00306
Volume14Issue:9Pages:3892-3899
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemical Research Methods
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordSOLID-PHASE EXTRACTION ; MASS-SPECTROMETRY ; QUANTITATIVE PROTEOMICS ; MAGNETIC NANOPARTICLES ; HYDRAZIDE CHEMISTRY ; REDUCTIVE AMINATION ; MESOPOROUS SILICA ; GLYCOSYLATION ; QUANTIFICATION ; OXIDATION
AbstractAn amine chemistry method was developed for the extraction of N-glycopeptides using amine-functionalized beads for glycoproteomics analysis. Two reductive amination reactions between primary amine and aldehyde were employed in this approach. The first one was to block the primary amines in the peptides by addition of formaldehyde and sodium c-yanoborohydride into the peptide sample, and the second one was to couple the glycopeptides onto solid phase beads by incubating the glycopeptides containing aldehyde groups (oxidized by periodate) with the amine-functionalized beads in the presence of sodium cyanoborohydride. It was demonstrated that the blocking of primary amines in the peptides by the first reductive amination reaction prior to the periodate oxidation made the amine chemistry method very efficient and sensitive. This new method was validated by analysis of glycoprotein standards as well as proteome samples. It was found that this new method led to significant increase in the identification of N-glycosites compared with the conventional hydrazide chemistry method.
Language英语
WOS IDWOS:000361087100042
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/146549
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Dalian Med Univ, Affiliated Hosp 2, Dalian 116027, Peoples R China
4.Jilin Univ, Res Ctr Drug Metab, Sch Life Sci, Changchun 130012, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Zhang,Sun, Deguang,Cong, Yuting,et al. Amine Chemistry Method for Selective Enrichment of N-Linked Glycopeptides for Glycoproteomics Analysis[J]. JOURNAL OF PROTEOME RESEARCH,2015,14(9):3892-3899.
APA Zhang, Zhang.,Sun, Deguang.,Cong, Yuting.,Mao, Jiawei.,Huang, Junfeng.,...&Zou, Hanfa.(2015).Amine Chemistry Method for Selective Enrichment of N-Linked Glycopeptides for Glycoproteomics Analysis.JOURNAL OF PROTEOME RESEARCH,14(9),3892-3899.
MLA Zhang, Zhang,et al."Amine Chemistry Method for Selective Enrichment of N-Linked Glycopeptides for Glycoproteomics Analysis".JOURNAL OF PROTEOME RESEARCH 14.9(2015):3892-3899.
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