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题名: Structural and functional analysis of a low-temperature-active alkaline esterase from South China Sea marine sediment microbial metagenomic library
作者: Hu, Yongfei1, 2, 3;  Liu, Yinghui4;  Li, Jing1, 2;  Feng, Yanbin4;  Lu, Na1, 2;  Zhu, Baoli1, 2, 3;  Xue, Song4
关键词: Marine sediment ;  Fosmid library ;  Esterase ;  Low-temperature active ;  Crystal structure
刊名: JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
发表日期: 2015-11-01
DOI: 10.1007/s10295-015-1653-2
卷: 42, 期:11, 页:1449-1461
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: A low-temperature-active alkaline esterase, Est12, from a marine sediment metagenomic fosmid library was identified. Est12 prefers short- and middle-chain p-nitrophenol esters as substrate with optimum temperature and pH value of 50 A degrees C and 9.0, respectively, and nearly 50 % of maximum activity retained at 5 A degrees C. The hydrolysis activity of Est12 was stable at 40 A degrees C. Ca2+ especially activated the activity of Est12 to about 151 % of the control. DEPC and PMSF inhibited the activity of Est12 to 34 and 25 %, respectively. In addition, Est12 was more tolerable to methanol compared to other organic solvents tested. The crystal structure of Est12 at 1.39 resolution showed that the cap domain which is composed of an alpha-helix and a flexible region resulted in a relatively wide spectrum of substrate, with p-nitrophenol caproate as the preferred one. Furthermore, the flexible cap domain and the high percentage of Gly, Ser, and Met may play important roles in the adaptation of Est12 to low temperature.
关键词[WOS]: ENVIRONMENTAL DNA LIBRARIES ;  INDUSTRIAL APPLICATIONS ;  UNCULTURED BACTERIA ;  LIPOLYTIC ENZYMES ;  ESCHERICHIA-COLI ;  LIPASE ;  IDENTIFICATION ;  GENES ;  FAMILY ;  MICROORGANISMS
语种: 英语
WOS记录号: WOS:000362964900003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/146600
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China
2.Beijing Key Lab Microbial Drug Resistance & Resis, Beijing 100101, Peoples R China
3.Zhejiang Univ, Coll Med, Affiliated Hosp 1, Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou 310006, Zhejiang, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioengn Grp, Dalian 116023, Peoples R China

Recommended Citation:
Hu, Yongfei,Liu, Yinghui,Li, Jing,et al. Structural and functional analysis of a low-temperature-active alkaline esterase from South China Sea marine sediment microbial metagenomic library[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2015,42(11):1449-1461.
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