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Surface sieving coordinated IMAC material for purification of His-tagged proteins
Li, Senwu1,2; Yang, Kaiguang1; Liu, Lukuan1,2,3; Zhao, Baofeng1; Chen, Yuanbo1,2; Li, Xiao1; Zhang, Lihua1; Zhang, Yukui1
KeywordSurface Sieving Metal Ion Affinity Protein Purification His-tag
Source PublicationANALYTICA CHIMICA ACTA
2017-10-29
DOI10.1016/j.aca.2017.10.021
Volume997Pages:9-15
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordION AFFINITY-CHROMATOGRAPHY ; RECOMBINANT PROTEINS ; MAGNETIC SEPARATION ; RAFT POLYMERIZATION ; PERSPECTIVES ; NANOPARTICLE ; ADSORPTION ; PROTEOMICS ; STRATEGY ; DELIVERY
AbstractTailor-made materials for the purification of proteins with His-tag was designed through synergizing the selectivity of surface sieving and metal ion affinity. By excluding impurity proteins out of the surface polymer network, such materials could purify His-tagged proteins from the crude cell lysis with purity up to 90%, improved by 14% compared to that obtained by the commercial metal chelating affinity materials. This study might promote the His-tagged protein purification to a new level. (c) 2017 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000415334500002
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/149669
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, CAS Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China
Recommended Citation
GB/T 7714
Li, Senwu,Yang, Kaiguang,Liu, Lukuan,et al. Surface sieving coordinated IMAC material for purification of His-tagged proteins[J]. ANALYTICA CHIMICA ACTA,2017,997:9-15.
APA Li, Senwu.,Yang, Kaiguang.,Liu, Lukuan.,Zhao, Baofeng.,Chen, Yuanbo.,...&Zhang, Yukui.(2017).Surface sieving coordinated IMAC material for purification of His-tagged proteins.ANALYTICA CHIMICA ACTA,997,9-15.
MLA Li, Senwu,et al."Surface sieving coordinated IMAC material for purification of His-tagged proteins".ANALYTICA CHIMICA ACTA 997(2017):9-15.
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