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题名: Novel Algorithm for Identification and Quantification of Proteins Based on Strategy of Isobaric Peptide Termini Labelling
作者: Zheng Nai-Ren1;  Shan Yi-Chu2;  Deng Yu-Lin1;  Zhang Yu-Kui1, 2
关键词: Proteomics ;  Isobaric labeling ;  Identification of proteins
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2017-10-01
DOI: 10.11895/j.issn.0253-3820.170250
卷: 45, 期:10, 页:1441-1447
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Isobaric peptide termini labeling (IPTL) is a technology which uses light and heavy isotopes to label C-terminus and N-terminus of peptides. As the masses of labeled peptides are equivalent, the complexity of sample is low when analyzing MS data produced by this technology. Besides, paired b and y ions are helpful while analyzing MS/MS data in this kind of experiments. On the basis of this, a novel scoring algorithm, all ions scoring algorithm (AISA), has been designed for IPTL experiments. The information of quantification and qualification can be acquired at the same time using AISA. On Q-Exactive HeLa 2D RPLC dataset, peptide spectrum matches (PSMs), distinct peptides and protein groups identified by AISA are 15%, 26% and 22% higher than Morpheus. On human-HCC-HL dataset, PSMs, distinct peptides and protein groups identified by AISA are 24%, 39% and 27% higher than Morpheus. Quantification ratio on Q-Exactive HeLa and human. HCC-HL datasets are 1.18 and 0.90, respectively, which are very close to 1. Besides, quantification ratios between 0.5 and 2.0 are 91% and 94%, respectively.
关键词[WOS]: TANDEM MASS TAGS ;  QUANTITATIVE PROTEOMICS ;  MS/MS ;  SPECTROMETRY ;  EXPRESSION ;  MIXTURES
语种: 英语
WOS记录号: WOS:000414957900004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/149672
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China

Recommended Citation:
Zheng Nai-Ren,Shan Yi-Chu,Deng Yu-Lin,et al. Novel Algorithm for Identification and Quantification of Proteins Based on Strategy of Isobaric Peptide Termini Labelling[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2017,45(10):1441-1447.
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