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Novel Algorithm for Identification and Quantification of Proteins Based on Strategy of Isobaric Peptide Termini Labelling
Zheng Nai-Ren1; Shan Yi-Chu2; Deng Yu-Lin1; Zhang Yu-Kui1,2
KeywordProteomics Isobaric Labeling Identification Of Proteins
Source PublicationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2017-10-01
DOI10.11895/j.issn.0253-3820.170250
Volume45Issue:10Pages:1441-1447
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordTANDEM MASS TAGS ; QUANTITATIVE PROTEOMICS ; MS/MS ; SPECTROMETRY ; EXPRESSION ; MIXTURES
AbstractIsobaric peptide termini labeling (IPTL) is a technology which uses light and heavy isotopes to label C-terminus and N-terminus of peptides. As the masses of labeled peptides are equivalent, the complexity of sample is low when analyzing MS data produced by this technology. Besides, paired b and y ions are helpful while analyzing MS/MS data in this kind of experiments. On the basis of this, a novel scoring algorithm, all ions scoring algorithm (AISA), has been designed for IPTL experiments. The information of quantification and qualification can be acquired at the same time using AISA. On Q-Exactive HeLa 2D RPLC dataset, peptide spectrum matches (PSMs), distinct peptides and protein groups identified by AISA are 15%, 26% and 22% higher than Morpheus. On human-HCC-HL dataset, PSMs, distinct peptides and protein groups identified by AISA are 24%, 39% and 27% higher than Morpheus. Quantification ratio on Q-Exactive HeLa and human. HCC-HL datasets are 1.18 and 0.90, respectively, which are very close to 1. Besides, quantification ratios between 0.5 and 2.0 are 91% and 94%, respectively.
Language英语
WOS IDWOS:000414957900004
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/149672
Collection中国科学院大连化学物理研究所
Affiliation1.Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Zheng Nai-Ren,Shan Yi-Chu,Deng Yu-Lin,et al. Novel Algorithm for Identification and Quantification of Proteins Based on Strategy of Isobaric Peptide Termini Labelling[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2017,45(10):1441-1447.
APA Zheng Nai-Ren,Shan Yi-Chu,Deng Yu-Lin,&Zhang Yu-Kui.(2017).Novel Algorithm for Identification and Quantification of Proteins Based on Strategy of Isobaric Peptide Termini Labelling.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,45(10),1441-1447.
MLA Zheng Nai-Ren,et al."Novel Algorithm for Identification and Quantification of Proteins Based on Strategy of Isobaric Peptide Termini Labelling".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 45.10(2017):1441-1447.
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