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A Method for Microalgae Proteomics Analysis Based on Modified Filter-Aided Sample Preparation
Li, Song1; Cao, Xupeng2; Wang, Yan3; Zhu, Zhen1; Zhang, Haowei1; Xue, Song2; Tian, Jing1
KeywordFasp Mfasp Chlamydomonas Reinhardtii Proteomics Sample Preparation
Source PublicationAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
2017-11-01
DOI10.1007/s12010-017-2473-9
Volume183Issue:3Pages:923-930
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS KeywordCHLAMYDOMONAS-REINHARDTII ; PROTEIN
AbstractWith the fast development of microalgal biofuel researches, the proteomics studies of microalgae increased quickly. A filter-aided sample preparation (FASP) method is widely used proteomics sample preparation method since 2009. Here, a method of microalgae proteomics analysis based on modified filter-aided sample preparation (mFASP) was described to meet the characteristics of microalgae cells and eliminate the error caused by over-alkylation. Using Chlamydomonas reinhardtii as the model, the prepared sample was tested by standard LC-MS/MS and compared with the previous reports. The results showed mFASP is suitable for most of occasions of microalgae proteomics studies.
Language英语
WOS IDWOS:000414195200017
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/149709
Collection中国科学院大连化学物理研究所
Affiliation1.Dalian Polytech Univ, Sch Bioengn, Dalian 116034, Peoples R China
2.Chinese Acad Sci, Marine Bioengn Grp, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Novel Technol & Mat Separat & Detect Biomol Grp, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Li, Song,Cao, Xupeng,Wang, Yan,et al. A Method for Microalgae Proteomics Analysis Based on Modified Filter-Aided Sample Preparation[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2017,183(3):923-930.
APA Li, Song.,Cao, Xupeng.,Wang, Yan.,Zhu, Zhen.,Zhang, Haowei.,...&Tian, Jing.(2017).A Method for Microalgae Proteomics Analysis Based on Modified Filter-Aided Sample Preparation.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,183(3),923-930.
MLA Li, Song,et al."A Method for Microalgae Proteomics Analysis Based on Modified Filter-Aided Sample Preparation".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 183.3(2017):923-930.
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