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题名: Surface sieving coordinated IMAC material for purification of His-tagged proteins
作者: Li, Senwu1, 2;  Yang, Kaiguang1;  Liu, Lukuan1, 2, 3;  Zhao, Baofeng1;  Chen, Yuanbo1, 2;  Li, Xiao1;  Zhang, Lihua1;  Zhang, Yukui1
关键词: Surface sieving ;  Metal ion affinity ;  Protein purification ;  His-tag
刊名: ANALYTICA CHIMICA ACTA
发表日期: 2017-10-29
DOI: 10.1016/j.aca.2017.10.021
卷: 997, 页:9-15
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Tailor-made materials for the purification of proteins with His-tag was designed through synergizing the selectivity of surface sieving and metal ion affinity. By excluding impurity proteins out of the surface polymer network, such materials could purify His-tagged proteins from the crude cell lysis with purity up to 90%, improved by 14% compared to that obtained by the commercial metal chelating affinity materials. This study might promote the His-tagged protein purification to a new level. (c) 2017 Elsevier B.V. All rights reserved.
关键词[WOS]: ION AFFINITY-CHROMATOGRAPHY ;  RECOMBINANT PROTEINS ;  MAGNETIC SEPARATION ;  RAFT POLYMERIZATION ;  PERSPECTIVES ;  NANOPARTICLE ;  ADSORPTION ;  PROTEOMICS ;  STRATEGY ;  DELIVERY
语种: 英语
WOS记录号: WOS:000415334500002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/149732
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, CAS Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China

Recommended Citation:
Li, Senwu,Yang, Kaiguang,Liu, Lukuan,et al. Surface sieving coordinated IMAC material for purification of His-tagged proteins[J]. ANALYTICA CHIMICA ACTA,2017,997:9-15.
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