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Novel Tetrapeptide Hydrophilic Interaction Chromatography Materials for High. efficiency Glycopeptide Enrichment
Chen Cheng1; Wang Hong-Xi2; Kang Hong-Jian1; Yao Yao2; Qing Guang-Yan2; Li Xiu-Ling1; Liang Xin-Miao1
KeywordHydrophilic Interaction Chromatography Tetrapeptide Glycopeptide Enrichment Mass Spectrometry
Source PublicationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2017-08-01
DOI10.11895/j.issn.0253-3820.170165
Volume45Issue:8Pages:1149-1154
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordN-LINKED GLYCOPEPTIDES ; SOLID-PHASE EXTRACTION ; MASS-SPECTROMETRY ; GLYCOSYLATION ; GLYCOPROTEINS ; HETEROGENEITY ; FETUIN
AbstractIn this study, a novel tetrapeptide hydrophilic interaction chromatography (HILIC) material was synthesized, and corresponding enrichment method for glycopeptides was developed. The tetrapeptide modified silica gel materials (denoted as Poly. DAPD) were synthesized by atom. transfer radical. polymerization (ATRP) and characterized by N-2 adsorption desorption and thermometer, thermal gravimetric analyzer (TGA) and X. ray photoelectron spectrometer (XPS). The characterization results indicated that tetrapeptide had been successfully synthesized on silica gel. Poly. DAPD materials showed high enrichment selectivity toward fetuin glycopeptides under solid. phase extraction (SPE) mode. Comparing with commercialized ZIC. HILIC in glycopeptides enrichment of fetuin digest which mixed with 5 mole ratio of albumin bovine (BSA), the as. prepared materials showed higher selectivity in both aspects of the identified number of glycopeptides and anti. interference property. The SPE results demonstrated that the tetrapeptide. based HILIC materials could be a potential tool in large. scale glycosylation analysis.
Language英语
WOS IDWOS:000414612900006
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/149737
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, CAS Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Wuhan Univ Technol, State Key Lab Adv Technol Mat Synth & Proc, Wuhan 430070, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Chen Cheng,Wang Hong-Xi,Kang Hong-Jian,et al. Novel Tetrapeptide Hydrophilic Interaction Chromatography Materials for High. efficiency Glycopeptide Enrichment[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2017,45(8):1149-1154.
APA Chen Cheng.,Wang Hong-Xi.,Kang Hong-Jian.,Yao Yao.,Qing Guang-Yan.,...&Liang Xin-Miao.(2017).Novel Tetrapeptide Hydrophilic Interaction Chromatography Materials for High. efficiency Glycopeptide Enrichment.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,45(8),1149-1154.
MLA Chen Cheng,et al."Novel Tetrapeptide Hydrophilic Interaction Chromatography Materials for High. efficiency Glycopeptide Enrichment".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 45.8(2017):1149-1154.
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