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A Method for Microalgae Proteomics Analysis Based on Modified Filter-Aided Sample Preparation
Li, Song1; Cao, Xupeng2; Wang, Yan3; Zhu, Zhen1; Zhang, Haowei1; Xue, Song2; Tian, Jing1
关键词Fasp Mfasp Chlamydomonas Reinhardtii Proteomics Sample Preparation
刊名APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
2017-11-01
DOI10.1007/s12010-017-2473-9
183期:3页:923-930
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]CHLAMYDOMONAS-REINHARDTII ; PROTEIN
英文摘要With the fast development of microalgal biofuel researches, the proteomics studies of microalgae increased quickly. A filter-aided sample preparation (FASP) method is widely used proteomics sample preparation method since 2009. Here, a method of microalgae proteomics analysis based on modified filter-aided sample preparation (mFASP) was described to meet the characteristics of microalgae cells and eliminate the error caused by over-alkylation. Using Chlamydomonas reinhardtii as the model, the prepared sample was tested by standard LC-MS/MS and compared with the previous reports. The results showed mFASP is suitable for most of occasions of microalgae proteomics studies.
语种英语
WOS记录号WOS:000414195200017
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/149772
专题中国科学院大连化学物理研究所
作者单位1.Dalian Polytech Univ, Sch Bioengn, Dalian 116034, Peoples R China
2.Chinese Acad Sci, Marine Bioengn Grp, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Novel Technol & Mat Separat & Detect Biomol Grp, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
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GB/T 7714
Li, Song,Cao, Xupeng,Wang, Yan,et al. A Method for Microalgae Proteomics Analysis Based on Modified Filter-Aided Sample Preparation[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2017,183(3):923-930.
APA Li, Song.,Cao, Xupeng.,Wang, Yan.,Zhu, Zhen.,Zhang, Haowei.,...&Tian, Jing.(2017).A Method for Microalgae Proteomics Analysis Based on Modified Filter-Aided Sample Preparation.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,183(3),923-930.
MLA Li, Song,et al."A Method for Microalgae Proteomics Analysis Based on Modified Filter-Aided Sample Preparation".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 183.3(2017):923-930.
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