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题名: A Method for Microalgae Proteomics Analysis Based on Modified Filter-Aided Sample Preparation
作者: Li, Song1;  Cao, Xupeng2;  Wang, Yan3;  Zhu, Zhen1;  Zhang, Haowei1;  Xue, Song2;  Tian, Jing1
关键词: FASP ;  mFASP ;  Chlamydomonas reinhardtii ;  Proteomics ;  Sample preparation
刊名: APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
发表日期: 2017-11-01
DOI: 10.1007/s12010-017-2473-9
卷: 183, 期:3, 页:923-930
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology
英文摘要: With the fast development of microalgal biofuel researches, the proteomics studies of microalgae increased quickly. A filter-aided sample preparation (FASP) method is widely used proteomics sample preparation method since 2009. Here, a method of microalgae proteomics analysis based on modified filter-aided sample preparation (mFASP) was described to meet the characteristics of microalgae cells and eliminate the error caused by over-alkylation. Using Chlamydomonas reinhardtii as the model, the prepared sample was tested by standard LC-MS/MS and compared with the previous reports. The results showed mFASP is suitable for most of occasions of microalgae proteomics studies.
关键词[WOS]: CHLAMYDOMONAS-REINHARDTII ;  PROTEIN
语种: 英语
WOS记录号: WOS:000414195200017
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/149772
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Polytech Univ, Sch Bioengn, Dalian 116034, Peoples R China
2.Chinese Acad Sci, Marine Bioengn Grp, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Novel Technol & Mat Separat & Detect Biomol Grp, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Li, Song,Cao, Xupeng,Wang, Yan,et al. A Method for Microalgae Proteomics Analysis Based on Modified Filter-Aided Sample Preparation[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2017,183(3):923-930.
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