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题名: Polymeric hydrophilic ionic liquids used to modify magnetic nanoparticles for the highly selective enrichment of N-linked glycopeptides
作者: Jiao, Fenglong1, 2;  Gao, Fangyuan2;  Wang, Heping2;  Deng, Yulin1;  Zhang, Yangjun2;  Qian, Xiaohong2;  Zhang, Yukui3
刊名: SCIENTIFIC REPORTS
发表日期: 2017-08-01
DOI: 10.1038/s41598-017-07516-x
卷: 7
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
英文摘要: The low abundance of glycopeptides in biological samples makes it necessary to enrich them before further analysis. In this study, the polymeric hydrophilic ionic liquid-modified magnetic (Fe3O4@MPS@PMAC) nanoparticles were synthesized via a one-step reflux-precipitation polymerization. Owing to the excellent hydrophilicity and strong electrostatic interaction toward glycopeptides of the polymerized hydrophilic ionic liquid, [2-(methacryloyloxy) ethyl] trimethylammonium chloride (MAC), the synthesized Fe3O4@MPS@PMAC nanoparticles exhibited outstanding performance in glycopeptide enrichment with high detection sensitivity (10 fmol), large binding capacity (100 mu g mg(-1)) and satisfied enrichment recovery (approximately 82%). Furthermore, the newly developed Fe3O4@MPS@PMAC nanoparticles were applied for the glycopeptide enrichment of HeLa exosome proteins. A total of 1274 glycopeptides from 536 glycoproteins were identified in three replicate analyses of 50 mu g of HeLa exosome proteins. These results demonstrate the potential of Fe3O4@MPS@PMAC nanoparticles for both glycoproteomic analysis and exosome research.
关键词[WOS]: PROTEIN GLYCOSYLATION ;  EFFICIENT ENRICHMENT ;  MASS-SPECTROMETRY ;  GLYCOPROTEINS ;  EXTRACTION ;  QUANTIFICATION ;  IDENTIFICATION ;  RECOGNITION ;  ADSORBENT ;  SAMPLES
语种: 英语
WOS记录号: WOS:000406764200016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/149794
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Beijing Inst Technol, Sch Life Sci & Technol, Beijing 100081, Peoples R China
2.Beijing Inst Radiat Med, State Key Lab Prote, Natl Ctr Prot Sci Beijing, Beijing 102200, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Jiao, Fenglong,Gao, Fangyuan,Wang, Heping,et al. Polymeric hydrophilic ionic liquids used to modify magnetic nanoparticles for the highly selective enrichment of N-linked glycopeptides[J]. SCIENTIFIC REPORTS,2017,7.
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