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Polymeric hydrophilic ionic liquids used to modify magnetic nanoparticles for the highly selective enrichment of N-linked glycopeptides
Jiao, Fenglong1,2; Gao, Fangyuan2; Wang, Heping2; Deng, Yulin1; Zhang, Yangjun2; Qian, Xiaohong2; Zhang, Yukui3
刊名SCIENTIFIC REPORTS
2017-08-01
DOI10.1038/s41598-017-07516-x
7
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]PROTEIN GLYCOSYLATION ; EFFICIENT ENRICHMENT ; MASS-SPECTROMETRY ; GLYCOPROTEINS ; EXTRACTION ; QUANTIFICATION ; IDENTIFICATION ; RECOGNITION ; ADSORBENT ; SAMPLES
英文摘要The low abundance of glycopeptides in biological samples makes it necessary to enrich them before further analysis. In this study, the polymeric hydrophilic ionic liquid-modified magnetic (Fe3O4@MPS@PMAC) nanoparticles were synthesized via a one-step reflux-precipitation polymerization. Owing to the excellent hydrophilicity and strong electrostatic interaction toward glycopeptides of the polymerized hydrophilic ionic liquid, [2-(methacryloyloxy) ethyl] trimethylammonium chloride (MAC), the synthesized Fe3O4@MPS@PMAC nanoparticles exhibited outstanding performance in glycopeptide enrichment with high detection sensitivity (10 fmol), large binding capacity (100 mu g mg(-1)) and satisfied enrichment recovery (approximately 82%). Furthermore, the newly developed Fe3O4@MPS@PMAC nanoparticles were applied for the glycopeptide enrichment of HeLa exosome proteins. A total of 1274 glycopeptides from 536 glycoproteins were identified in three replicate analyses of 50 mu g of HeLa exosome proteins. These results demonstrate the potential of Fe3O4@MPS@PMAC nanoparticles for both glycoproteomic analysis and exosome research.
语种英语
WOS记录号WOS:000406764200016
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/149794
专题中国科学院大连化学物理研究所
作者单位1.Beijing Inst Technol, Sch Life Sci & Technol, Beijing 100081, Peoples R China
2.Beijing Inst Radiat Med, State Key Lab Prote, Natl Ctr Prot Sci Beijing, Beijing 102200, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
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GB/T 7714
Jiao, Fenglong,Gao, Fangyuan,Wang, Heping,et al. Polymeric hydrophilic ionic liquids used to modify magnetic nanoparticles for the highly selective enrichment of N-linked glycopeptides[J]. SCIENTIFIC REPORTS,2017,7.
APA Jiao, Fenglong.,Gao, Fangyuan.,Wang, Heping.,Deng, Yulin.,Zhang, Yangjun.,...&Zhang, Yukui.(2017).Polymeric hydrophilic ionic liquids used to modify magnetic nanoparticles for the highly selective enrichment of N-linked glycopeptides.SCIENTIFIC REPORTS,7.
MLA Jiao, Fenglong,et al."Polymeric hydrophilic ionic liquids used to modify magnetic nanoparticles for the highly selective enrichment of N-linked glycopeptides".SCIENTIFIC REPORTS 7(2017).
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