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An Optimized Two-Photon Fluorescent Probe for Biological Sensing and Imaging of Catechol-O-Methyltransferase
Wang, Ping1; Xia, Yang-Liu2; Zou, Li-Wei1; Qian, Xing-Kai2; Dou, Tong-Yi2; Jin, Qiang1; Li, Shi-Yang2; Yu, Yang2; Wang, Dan-Dan1; Luo, Qun3; Ge, Guang-Bo1,2; Yang, Ling1
KeywordBio-imaging Biosensing Catechol-o-methyltransferase Coumarins Fluorescence
Source PublicationCHEMISTRY-A EUROPEAN JOURNAL
2017-08-10
DOI10.1002/chem.201701384
Volume23Issue:45Pages:10800-10807
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordNITRIC-OXIDE ; PARKINSONS-DISEASE ; LIVING CELLS ; METHYLATION ; DESIGN ; INHIBITORS ; COMT ; SCHIZOPHRENIA ; POLYMORPHISM ; DISORDERS
AbstractA practical two-photon fluorescent probe was developed for highly sensitive and selective sensing of the activities of catechol-O-methyltransferase (COMT) in complex biological samples. To this end, a series of 3-substituted 7,8-dihydroxycoumarins were designed and synthesized. Among them, 3-BTD displayed the best combination of selectivity, sensitivity, reactivity, and fluorescence response following COMT-catalyzed 8-O-methylation. The newly developed two-photon fluorescent probe 3-BTD can be used for determining the activities of COMT in complex biological samples and bio-imaging of endogenous COMT in living cells and tissue slices with good cell permeability, low cytotoxicity, and high imaging resolution. All these findings suggest that 3-BTD holds great promise for developing therapeutic molecules that target COMT, as well as for exploring COMT-associated biological processes and its biological functions in living systems. Furthermore, the strategy also sheds new light on the development of fluorescent probes for other conjugative enzymes.
Language英语
WOS IDWOS:000407397300015
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/149799
Collection中国科学院大连化学物理研究所
Affiliation1.Shanghai Univ Tradit Chinese Med, Shanghai 201203, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Natl Ctr Mass Spectrometry Beijing, CAS Key Lab Analyt Chem Living Biosyst,Inst Chem, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Wang, Ping,Xia, Yang-Liu,Zou, Li-Wei,et al. An Optimized Two-Photon Fluorescent Probe for Biological Sensing and Imaging of Catechol-O-Methyltransferase[J]. CHEMISTRY-A EUROPEAN JOURNAL,2017,23(45):10800-10807.
APA Wang, Ping.,Xia, Yang-Liu.,Zou, Li-Wei.,Qian, Xing-Kai.,Dou, Tong-Yi.,...&Yang, Ling.(2017).An Optimized Two-Photon Fluorescent Probe for Biological Sensing and Imaging of Catechol-O-Methyltransferase.CHEMISTRY-A EUROPEAN JOURNAL,23(45),10800-10807.
MLA Wang, Ping,et al."An Optimized Two-Photon Fluorescent Probe for Biological Sensing and Imaging of Catechol-O-Methyltransferase".CHEMISTRY-A EUROPEAN JOURNAL 23.45(2017):10800-10807.
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