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题名: An Optimized Two-Photon Fluorescent Probe for Biological Sensing and Imaging of Catechol-O-Methyltransferase
作者: Wang, Ping1;  Xia, Yang-Liu2;  Zou, Li-Wei1;  Qian, Xing-Kai2;  Dou, Tong-Yi2;  Jin, Qiang1;  Li, Shi-Yang2;  Yu, Yang2;  Wang, Dan-Dan1;  Luo, Qun3;  Ge, Guang-Bo1, 2;  Yang, Ling1
关键词: bio-imaging ;  biosensing ;  catechol-o-methyltransferase ;  coumarins ;  fluorescence
刊名: CHEMISTRY-A EUROPEAN JOURNAL
发表日期: 2017-08-10
DOI: 10.1002/chem.201701384
卷: 23, 期:45, 页:10800-10807
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: A practical two-photon fluorescent probe was developed for highly sensitive and selective sensing of the activities of catechol-O-methyltransferase (COMT) in complex biological samples. To this end, a series of 3-substituted 7,8-dihydroxycoumarins were designed and synthesized. Among them, 3-BTD displayed the best combination of selectivity, sensitivity, reactivity, and fluorescence response following COMT-catalyzed 8-O-methylation. The newly developed two-photon fluorescent probe 3-BTD can be used for determining the activities of COMT in complex biological samples and bio-imaging of endogenous COMT in living cells and tissue slices with good cell permeability, low cytotoxicity, and high imaging resolution. All these findings suggest that 3-BTD holds great promise for developing therapeutic molecules that target COMT, as well as for exploring COMT-associated biological processes and its biological functions in living systems. Furthermore, the strategy also sheds new light on the development of fluorescent probes for other conjugative enzymes.
关键词[WOS]: NITRIC-OXIDE ;  PARKINSONS-DISEASE ;  LIVING CELLS ;  METHYLATION ;  DESIGN ;  INHIBITORS ;  COMT ;  SCHIZOPHRENIA ;  POLYMORPHISM ;  DISORDERS
语种: 英语
WOS记录号: WOS:000407397300015
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/149799
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Shanghai Univ Tradit Chinese Med, Shanghai 201203, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Natl Ctr Mass Spectrometry Beijing, CAS Key Lab Analyt Chem Living Biosyst,Inst Chem, Beijing 100190, Peoples R China

Recommended Citation:
Wang, Ping,Xia, Yang-Liu,Zou, Li-Wei,et al. An Optimized Two-Photon Fluorescent Probe for Biological Sensing and Imaging of Catechol-O-Methyltransferase[J]. CHEMISTRY-A EUROPEAN JOURNAL,2017,23(45):10800-10807.
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