DICP OpenIR
4 '-Hydroxywogonin suppresses lipopolysaccharide-induced inflammatory responses in RAW 264.7 macrophages and acute lung injury mice
Fan, Chao1; Wu, Le-Hao1; Zhang, Gu-Fang1; Xu, Fangfang2; Zhang, Shuo1; Zhang, Xiuli2; Sun, Lei1; Yu, Yang1; Zhang, Yan1; Ye, Richard D.1,3
刊名PLOS ONE
2017-08-08
DOI10.1371/journal.pone.0181191
12期:8
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]NF-KAPPA-B ; ANTIINFLAMMATORY ACTIVITY ; PROTEIN-KINASES ; GENE-EXPRESSION ; P38 MAPK ; WOGONIN ; ACTIVATION ; PATHWAYS ; PHOSPHORYLATION ; ALPHA
英文摘要4'-Hydroxywogonin (4'-HW), a flavonoid, has been isolated from various plants and shown to inhibit NO production in macrophages. However, the molecular mechanisms and its in vivo activity have not been determined. Our study aimed to investigate the mechanisms underlying the anti-inflammatory effects of 4'-HW in vitro and in vivo. We showed that 4'-HW potently reduced the expression levels of COX-2 and iNOS as well as their products, prostaglandin E2 (PGE2) and nitric oxide (NO) respectively, in LPS-stimulated RAW 264.7 macrophages. 4'-HW also suppressed LPS-induced pro-inflammatory cytokines at mRNA and protein levels. Moreover, 4'-HW blocked the interaction of TAK1 and TAB1 in LPS-stimulated RAW 264.7 macrophages, resulting in an inhibition of the TAK1/IKK/NF-kappa B signaling pathway. Furthermore, 4'-HW also reduced the phosphorylation of MAPKs and PI3/Akt signaling pathways in LPS-stimulated RAW 264.7 macrophages. 4'-HW was also significantly decreased the intracellular reactive oxygen species (ROS) level. The effect of 4'-HW was confirmed in vivo. 4'-HW exhibited potent protective effect against LPS-induced ALI in mice. These findings indicate that 4'-HW suppresses the LPS-induced response in vitro and in vivo. It is likely that the inhibition of the TAK1/IKK/NF-kappa B, MAPKs and PI3/AKT signaling pathways contribute to the anti-inflammatory effects of 4'-HW. Our study suggests that 4'-HW may be an important functional constituent in the plants and has the potential value to be developed as a novel anti-inflammatory agent.
语种英语
WOS记录号WOS:000407144700004
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/149830
专题中国科学院大连化学物理研究所
作者单位1.Shanghai Jiao Tong Univ, Sch Pharm, Shanghai, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
3.Univ Macau, Inst Chinese Med Sci, Macau, Peoples R China
推荐引用方式
GB/T 7714
Fan, Chao,Wu, Le-Hao,Zhang, Gu-Fang,et al. 4 '-Hydroxywogonin suppresses lipopolysaccharide-induced inflammatory responses in RAW 264.7 macrophages and acute lung injury mice[J]. PLOS ONE,2017,12(8).
APA Fan, Chao.,Wu, Le-Hao.,Zhang, Gu-Fang.,Xu, Fangfang.,Zhang, Shuo.,...&Ye, Richard D..(2017).4 '-Hydroxywogonin suppresses lipopolysaccharide-induced inflammatory responses in RAW 264.7 macrophages and acute lung injury mice.PLOS ONE,12(8).
MLA Fan, Chao,et al."4 '-Hydroxywogonin suppresses lipopolysaccharide-induced inflammatory responses in RAW 264.7 macrophages and acute lung injury mice".PLOS ONE 12.8(2017).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Fan, Chao]的文章
[Wu, Le-Hao]的文章
[Zhang, Gu-Fang]的文章
百度学术
百度学术中相似的文章
[Fan, Chao]的文章
[Wu, Le-Hao]的文章
[Zhang, Gu-Fang]的文章
必应学术
必应学术中相似的文章
[Fan, Chao]的文章
[Wu, Le-Hao]的文章
[Zhang, Gu-Fang]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。