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Next-generation transgenic cotton: pyramiding RNAi and Bt counters insect resistance
Ni, Mi1,2; Ma, Wei3; Wang, Xiaofang1,2; Gao, Meijing4; Dai, Yan1,2; Wei, Xiaoli1,2; Zhang, Lei1,2; Peng, Yonggang1,2; Chen, Shuyuan1,2; Ding, Lingyun3; Tian, Yue3; Li, Jie3; Wang, Haiping3; Wang, Xiaolin5; Xu, Guowang5; Guo, Wangzhen3; Yang, Yihua4; Wu, Yidong4; Heuberger, Shannon6; Tabashnik, Bruce E.6; Zhang, Tianzhen3; Zhu, Zhen1,2
KeywordGenetic Engineering Rna Interference Bacillus Thuringiensis Juvenile Hormone Helicoverpa Armigera Sustainability
Source PublicationPLANT BIOTECHNOLOGY JOURNAL
2017-09-01
DOI10.1111/pbi.12709
Volume15Issue:9Pages:1204-1213
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiotechnology & Applied Microbiology ; Plant Sciences
WOS Research AreaBiotechnology & Applied Microbiology ; Plant Sciences
WOS KeywordFIELD-EVOLVED RESISTANCE ; WESTERN CORN-ROOTWORM ; DIABROTICA-VIRGIFERA-VIRGIFERA ; BACILLUS-THURINGIENSIS TOXINS ; JUVENILE-HORMONE ; HELICOVERPA-ARMIGERA ; PEST-MANAGEMENT ; CROPS ; GENE ; BOLLWORM
AbstractTransgenic crops producing insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) are extensively cultivated worldwide. To counter rapidly increasing pest resistance to crops that produce single Bt toxins, transgenic plant 'pyramids' producing two or more Bt toxins that kill the same pest have been widely adopted. However, cross-resistance and antagonism between Bt toxins limit the sustainability of this approach. Here we describe development and testing of the first pyramids of cotton combining protection from a Bt toxin and RNA interference (RNAi). We developed two types of transgenic cotton plants producing double-stranded RNA (dsRNA) from the global lepidopteran pest Helicoverpa armigera designed to interfere with its metabolism of juvenile hormone (JH). We focused on suppression of JH acid methyltransferase (JHAMT), which is crucial for JH synthesis, and JH-binding protein (JHBP), which transports JH to organs. In 2015 and 2016, we tested larvae from a Bt-resistant strain and a related susceptible strain of H. armigera on seven types of cotton: two controls, Bt cotton, two types of RNAi cotton (targeting JHAMT or JHBP) and two pyramids (Bt cotton plus each type of RNAi). Both types of RNAi cotton were effective against Bt-resistant insects. Bt cotton and RNAi acted independently against the susceptible strain. In computer simulations of conditions in northern China, where millions of farmers grow Bt cotton as well as abundant non-transgenic host plants of H. armigera, pyramided cotton combining a Bt toxin and RNAi substantially delayed resistance relative to using Bt cotton alone.
Language英语
WOS IDWOS:000407378100011
Citation statistics
Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/149840
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, State Key Lab Plant Genom, Beijing, Peoples R China
2.Chinese Acad Sci, Natl Ctr Plant Gene Res Beijing, Inst Genet & Dev Biol, Beijing, Peoples R China
3.Nanjing Agr Univ, Natl Key Lab Crop Genet & Germplasm Enhancement, Jiangsu Plant Gene Engn Res Ctr, Nanjing, Jiangsu, Peoples R China
4.Nanjing Agr Univ, Coll Plant Protect, Nanjing, Jiangsu, Peoples R China
5.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian, Peoples R China
6.Univ Arizona, Dept Entomol, Tucson, AZ 85721 USA
Recommended Citation
GB/T 7714
Ni, Mi,Ma, Wei,Wang, Xiaofang,et al. Next-generation transgenic cotton: pyramiding RNAi and Bt counters insect resistance[J]. PLANT BIOTECHNOLOGY JOURNAL,2017,15(9):1204-1213.
APA Ni, Mi.,Ma, Wei.,Wang, Xiaofang.,Gao, Meijing.,Dai, Yan.,...&Zhu, Zhen.(2017).Next-generation transgenic cotton: pyramiding RNAi and Bt counters insect resistance.PLANT BIOTECHNOLOGY JOURNAL,15(9),1204-1213.
MLA Ni, Mi,et al."Next-generation transgenic cotton: pyramiding RNAi and Bt counters insect resistance".PLANT BIOTECHNOLOGY JOURNAL 15.9(2017):1204-1213.
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