DICP OpenIR
Next-generation transgenic cotton: pyramiding RNAi and Bt counters insect resistance
Ni, Mi1,2; Ma, Wei3; Wang, Xiaofang1,2; Gao, Meijing4; Dai, Yan1,2; Wei, Xiaoli1,2; Zhang, Lei1,2; Peng, Yonggang1,2; Chen, Shuyuan1,2; Ding, Lingyun3; Tian, Yue3; Li, Jie3; Wang, Haiping3; Wang, Xiaolin5; Xu, Guowang5; Guo, Wangzhen3; Yang, Yihua4; Wu, Yidong4; Heuberger, Shannon6; Tabashnik, Bruce E.6; Zhang, Tianzhen3; Zhu, Zhen1,2
关键词Genetic Engineering Rna Interference Bacillus Thuringiensis Juvenile Hormone Helicoverpa Armigera Sustainability
刊名PLANT BIOTECHNOLOGY JOURNAL
2017-09-01
DOI10.1111/pbi.12709
15期:9页:1204-1213
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology ; Plant Sciences
研究领域[WOS]Biotechnology & Applied Microbiology ; Plant Sciences
关键词[WOS]FIELD-EVOLVED RESISTANCE ; WESTERN CORN-ROOTWORM ; DIABROTICA-VIRGIFERA-VIRGIFERA ; BACILLUS-THURINGIENSIS TOXINS ; JUVENILE-HORMONE ; HELICOVERPA-ARMIGERA ; PEST-MANAGEMENT ; CROPS ; GENE ; BOLLWORM
英文摘要Transgenic crops producing insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) are extensively cultivated worldwide. To counter rapidly increasing pest resistance to crops that produce single Bt toxins, transgenic plant 'pyramids' producing two or more Bt toxins that kill the same pest have been widely adopted. However, cross-resistance and antagonism between Bt toxins limit the sustainability of this approach. Here we describe development and testing of the first pyramids of cotton combining protection from a Bt toxin and RNA interference (RNAi). We developed two types of transgenic cotton plants producing double-stranded RNA (dsRNA) from the global lepidopteran pest Helicoverpa armigera designed to interfere with its metabolism of juvenile hormone (JH). We focused on suppression of JH acid methyltransferase (JHAMT), which is crucial for JH synthesis, and JH-binding protein (JHBP), which transports JH to organs. In 2015 and 2016, we tested larvae from a Bt-resistant strain and a related susceptible strain of H. armigera on seven types of cotton: two controls, Bt cotton, two types of RNAi cotton (targeting JHAMT or JHBP) and two pyramids (Bt cotton plus each type of RNAi). Both types of RNAi cotton were effective against Bt-resistant insects. Bt cotton and RNAi acted independently against the susceptible strain. In computer simulations of conditions in northern China, where millions of farmers grow Bt cotton as well as abundant non-transgenic host plants of H. armigera, pyramided cotton combining a Bt toxin and RNAi substantially delayed resistance relative to using Bt cotton alone.
语种英语
WOS记录号WOS:000407378100011
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/149840
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, State Key Lab Plant Genom, Beijing, Peoples R China
2.Chinese Acad Sci, Natl Ctr Plant Gene Res Beijing, Inst Genet & Dev Biol, Beijing, Peoples R China
3.Nanjing Agr Univ, Natl Key Lab Crop Genet & Germplasm Enhancement, Jiangsu Plant Gene Engn Res Ctr, Nanjing, Jiangsu, Peoples R China
4.Nanjing Agr Univ, Coll Plant Protect, Nanjing, Jiangsu, Peoples R China
5.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian, Peoples R China
6.Univ Arizona, Dept Entomol, Tucson, AZ 85721 USA
推荐引用方式
GB/T 7714
Ni, Mi,Ma, Wei,Wang, Xiaofang,et al. Next-generation transgenic cotton: pyramiding RNAi and Bt counters insect resistance[J]. PLANT BIOTECHNOLOGY JOURNAL,2017,15(9):1204-1213.
APA Ni, Mi.,Ma, Wei.,Wang, Xiaofang.,Gao, Meijing.,Dai, Yan.,...&Zhu, Zhen.(2017).Next-generation transgenic cotton: pyramiding RNAi and Bt counters insect resistance.PLANT BIOTECHNOLOGY JOURNAL,15(9),1204-1213.
MLA Ni, Mi,et al."Next-generation transgenic cotton: pyramiding RNAi and Bt counters insect resistance".PLANT BIOTECHNOLOGY JOURNAL 15.9(2017):1204-1213.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Ni, Mi]的文章
[Ma, Wei]的文章
[Wang, Xiaofang]的文章
百度学术
百度学术中相似的文章
[Ni, Mi]的文章
[Ma, Wei]的文章
[Wang, Xiaofang]的文章
必应学术
必应学术中相似的文章
[Ni, Mi]的文章
[Ma, Wei]的文章
[Wang, Xiaofang]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。