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题名: Next-generation transgenic cotton: pyramiding RNAi and Bt counters insect resistance
作者: Ni, Mi1, 2;  Ma, Wei3;  Wang, Xiaofang1, 2;  Gao, Meijing4;  Dai, Yan1, 2;  Wei, Xiaoli1, 2;  Zhang, Lei1, 2;  Peng, Yonggang1, 2;  Chen, Shuyuan1, 2;  Ding, Lingyun3;  Tian, Yue3;  Li, Jie3;  Wang, Haiping3;  Wang, Xiaolin5;  Xu, Guowang5;  Guo, Wangzhen3;  Yang, Yihua4;  Wu, Yidong4;  Heuberger, Shannon6;  Tabashnik, Bruce E.6;  Zhang, Tianzhen3;  Zhu, Zhen1, 2
关键词: genetic engineering ;  RNA interference ;  Bacillus thuringiensis ;  juvenile hormone ;  Helicoverpa armigera ;  sustainability
刊名: PLANT BIOTECHNOLOGY JOURNAL
发表日期: 2017-09-01
DOI: 10.1111/pbi.12709
卷: 15, 期:9, 页:1204-1213
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology ;  Plant Sciences
研究领域[WOS]: Biotechnology & Applied Microbiology ;  Plant Sciences
英文摘要: Transgenic crops producing insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) are extensively cultivated worldwide. To counter rapidly increasing pest resistance to crops that produce single Bt toxins, transgenic plant 'pyramids' producing two or more Bt toxins that kill the same pest have been widely adopted. However, cross-resistance and antagonism between Bt toxins limit the sustainability of this approach. Here we describe development and testing of the first pyramids of cotton combining protection from a Bt toxin and RNA interference (RNAi). We developed two types of transgenic cotton plants producing double-stranded RNA (dsRNA) from the global lepidopteran pest Helicoverpa armigera designed to interfere with its metabolism of juvenile hormone (JH). We focused on suppression of JH acid methyltransferase (JHAMT), which is crucial for JH synthesis, and JH-binding protein (JHBP), which transports JH to organs. In 2015 and 2016, we tested larvae from a Bt-resistant strain and a related susceptible strain of H. armigera on seven types of cotton: two controls, Bt cotton, two types of RNAi cotton (targeting JHAMT or JHBP) and two pyramids (Bt cotton plus each type of RNAi). Both types of RNAi cotton were effective against Bt-resistant insects. Bt cotton and RNAi acted independently against the susceptible strain. In computer simulations of conditions in northern China, where millions of farmers grow Bt cotton as well as abundant non-transgenic host plants of H. armigera, pyramided cotton combining a Bt toxin and RNAi substantially delayed resistance relative to using Bt cotton alone.
关键词[WOS]: FIELD-EVOLVED RESISTANCE ;  WESTERN CORN-ROOTWORM ;  DIABROTICA-VIRGIFERA-VIRGIFERA ;  BACILLUS-THURINGIENSIS TOXINS ;  JUVENILE-HORMONE ;  HELICOVERPA-ARMIGERA ;  PEST-MANAGEMENT ;  CROPS ;  GENE ;  BOLLWORM
语种: 英语
WOS记录号: WOS:000407378100011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/149840
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, State Key Lab Plant Genom, Beijing, Peoples R China
2.Chinese Acad Sci, Natl Ctr Plant Gene Res Beijing, Inst Genet & Dev Biol, Beijing, Peoples R China
3.Nanjing Agr Univ, Natl Key Lab Crop Genet & Germplasm Enhancement, Jiangsu Plant Gene Engn Res Ctr, Nanjing, Jiangsu, Peoples R China
4.Nanjing Agr Univ, Coll Plant Protect, Nanjing, Jiangsu, Peoples R China
5.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian, Peoples R China
6.Univ Arizona, Dept Entomol, Tucson, AZ 85721 USA

Recommended Citation:
Ni, Mi,Ma, Wei,Wang, Xiaofang,et al. Next-generation transgenic cotton: pyramiding RNAi and Bt counters insect resistance[J]. PLANT BIOTECHNOLOGY JOURNAL,2017,15(9):1204-1213.
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