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题名: Inert Gas Deactivates Protein Activity by Aggregation
作者: Zhang, Lijuan1, 2;  Zhang, Yuebin3;  Cheng, Jie1, 5, 6;  Wang, Lei2, 6, 7;  Wang, Xingya2, 6;  Zhang, Meng1, 4;  Gao, Yi1, 4;  Hu, Jun1, 5;  Zhang, Xuehua8;  Lu, Junhong1, 5;  Li, Guohui3;  Tai, Renzhong1, 2;  Fang, Haiping1, 4
刊名: SCIENTIFIC REPORTS
发表日期: 2017-08-31
DOI: 10.1038/s41598-017-10678-3
卷: 7
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
英文摘要: Biologically inert gases play important roles in the biological functionality of proteins. However, researchers lack a full understanding of the effects of these gases since they are very chemically stable only weakly absorbed by biological tissues. By combining X-ray fluorescence, particle sizing and molecular dynamics (MD) simulations, this work shows that the aggregation of these inert gases near the hydrophobic active cavity of pepsin should lead to protein deactivation. Micro X-ray fluorescence spectra show that a pepsin solution can contain a high concentration of Xe or Kr after gassing, and that the gas concentrations decrease quickly with degassing time. Biological activity experiments indicate a reversible deactivation of the protein during this gassing and degassing. Meanwhile, the nanoparticle size measurements reveal a higher number of "nanoparticles" in gas-containing pepsin solution, also supporting the possible interaction between inert gases and the protein. Further, MD simulations indicate that gas molecules can aggregate into a tiny bubble shape near the hydrophobic active cavity of pepsin, suggesting a mechanism for reducing their biological function.
关键词[WOS]: DER-WAALS COMPLEXES ;  XENON ;  BINDING ;  XE ;  MYOGLOBIN ;  PATHWAYS ;  NITROGEN ;  CAVITY ;  KR ;  IONIZATION
语种: 英语
WOS记录号: WOS:000408781200035
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/149879
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Key Lab Interfacial Phys & Technol, Shanghai 201800, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Shanghai 201204, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Dalian 116023, Peoples R China
4.Chinese Acad Sci, Shanghai Inst Appl Phys, Div Interfacial Water, Shanghai 201800, Peoples R China
5.Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China
6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
7.Cent South Univ Forestry & Technol, Inst Math & Phys, Changsha 410004, Hunan, Peoples R China
8.RMIT Univ, Sch Engn, Soft Matter & Interfaces Grp, Melbourne, Vic 3001, Australia

Recommended Citation:
Zhang, Lijuan,Zhang, Yuebin,Cheng, Jie,et al. Inert Gas Deactivates Protein Activity by Aggregation[J]. SCIENTIFIC REPORTS,2017,7.
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