DICP OpenIR
Inert Gas Deactivates Protein Activity by Aggregation
Zhang, Lijuan1,2; Zhang, Yuebin3; Cheng, Jie1,5,6; Wang, Lei2,6,7; Wang, Xingya2,6; Zhang, Meng1,4; Gao, Yi1,4; Hu, Jun1,5; Zhang, Xuehua8; Lu, Junhong1,5; Li, Guohui3; Tai, Renzhong1,2; Fang, Haiping1,4
Source PublicationSCIENTIFIC REPORTS
2017-08-31
DOI10.1038/s41598-017-10678-3
Volume7
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology
WOS SubjectMultidisciplinary Sciences
WOS Research AreaScience & Technology - Other Topics
WOS KeywordDER-WAALS COMPLEXES ; XENON ; BINDING ; XE ; MYOGLOBIN ; PATHWAYS ; NITROGEN ; CAVITY ; KR ; IONIZATION
AbstractBiologically inert gases play important roles in the biological functionality of proteins. However, researchers lack a full understanding of the effects of these gases since they are very chemically stable only weakly absorbed by biological tissues. By combining X-ray fluorescence, particle sizing and molecular dynamics (MD) simulations, this work shows that the aggregation of these inert gases near the hydrophobic active cavity of pepsin should lead to protein deactivation. Micro X-ray fluorescence spectra show that a pepsin solution can contain a high concentration of Xe or Kr after gassing, and that the gas concentrations decrease quickly with degassing time. Biological activity experiments indicate a reversible deactivation of the protein during this gassing and degassing. Meanwhile, the nanoparticle size measurements reveal a higher number of "nanoparticles" in gas-containing pepsin solution, also supporting the possible interaction between inert gases and the protein. Further, MD simulations indicate that gas molecules can aggregate into a tiny bubble shape near the hydrophobic active cavity of pepsin, suggesting a mechanism for reducing their biological function.
Language英语
WOS IDWOS:000408781200035
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/149879
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Key Lab Interfacial Phys & Technol, Shanghai 201800, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Shanghai 201204, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Dalian 116023, Peoples R China
4.Chinese Acad Sci, Shanghai Inst Appl Phys, Div Interfacial Water, Shanghai 201800, Peoples R China
5.Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China
6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
7.Cent South Univ Forestry & Technol, Inst Math & Phys, Changsha 410004, Hunan, Peoples R China
8.RMIT Univ, Sch Engn, Soft Matter & Interfaces Grp, Melbourne, Vic 3001, Australia
Recommended Citation
GB/T 7714
Zhang, Lijuan,Zhang, Yuebin,Cheng, Jie,et al. Inert Gas Deactivates Protein Activity by Aggregation[J]. SCIENTIFIC REPORTS,2017,7.
APA Zhang, Lijuan.,Zhang, Yuebin.,Cheng, Jie.,Wang, Lei.,Wang, Xingya.,...&Fang, Haiping.(2017).Inert Gas Deactivates Protein Activity by Aggregation.SCIENTIFIC REPORTS,7.
MLA Zhang, Lijuan,et al."Inert Gas Deactivates Protein Activity by Aggregation".SCIENTIFIC REPORTS 7(2017).
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