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Characterization the carotenoid productions and profiles of three Rhodosporidium toruloides mutants from Agrobacterium tumefaciens-mediated transformation
Lin, Xinping1; Gao, Ning1; Liu, Sasa1; Zhang, Sufang2; Song, Shuang1; Ji, Chaofan1; Dong, Xiuping1; Su, Yichen3; Zhao, Zongbao Kent2; Zhu, Beiwei1
KeywordRhodosporidium Toruloides Carotenoids Agrobacterium Tumefaciens-mediated Transformation T-dna Insertion Mutants
Source PublicationYEAST
2017-08-01
DOI10.1002/yea.3236
Volume34Issue:8Pages:335-342
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Microbiology ; Mycology
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Microbiology ; Mycology
WOS KeywordBY-PRODUCTS ; RHODOTORULA ; YEASTS ; EFFICIENCY ; GLYCEROL ; LIPIDS ; FOOD
AbstractThe red yeast Rhodosporidium toruloides is a known lipid producer capable of accumulating large amounts of triacylglycerols and carotenoids. However, it remains challenging to study its carotenoid production profiles owing to limited biochemical information and inefficient genetic tools. Here we used an Agrobacterium tumefaciensmediated transformation (ATMT) to change its carotenoid production and profiles. We constructed R. toruloides NP11 mutant libraries with ATMT, selected three mutants with different colours, characterized their carotenoid products by high-pressure liquid chromatography-mass spectrometry (HPLC-MS) analysis and assured differences among those strains in terms of carotenoid production and its composition profiles. We then located T-DNA insertion sites using the genome walking technology and provided discussions in terms of the new phenotypes. This study is the first of its kind to change the carotenoid production profiles in R. toruloides. Copyright (C) 2017 John Wiley & Sons, Ltd.
Language英语
WOS IDWOS:000409009500002
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/149881
Collection中国科学院大连化学物理研究所
Affiliation1.Dalian Polytech Univ, Natl Engn Res Ctr Seafood, Sch Food Sci & Technol, Dalian 116034, Peoples R China
2.Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
3.Oregon State Univ, Seafood Res & Educ Ctr, Astoria, OR 97103 USA
Recommended Citation
GB/T 7714
Lin, Xinping,Gao, Ning,Liu, Sasa,et al. Characterization the carotenoid productions and profiles of three Rhodosporidium toruloides mutants from Agrobacterium tumefaciens-mediated transformation[J]. YEAST,2017,34(8):335-342.
APA Lin, Xinping.,Gao, Ning.,Liu, Sasa.,Zhang, Sufang.,Song, Shuang.,...&Zhu, Beiwei.(2017).Characterization the carotenoid productions and profiles of three Rhodosporidium toruloides mutants from Agrobacterium tumefaciens-mediated transformation.YEAST,34(8),335-342.
MLA Lin, Xinping,et al."Characterization the carotenoid productions and profiles of three Rhodosporidium toruloides mutants from Agrobacterium tumefaciens-mediated transformation".YEAST 34.8(2017):335-342.
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